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• W2011714229 abstract "In this work, we evaluated the expansion of human hematopoietic stem cells from umbilical cord blood in roller bottles. The Iscove's modified Dulbecco's medium, the Stem Pro 34-SFM medium, and the L-15 Leibovitz's medium for cultures in CO2-free atmosphere were assessed. At day 5 of culture, total colony forming unit expansions of 14.44 ± 3.74, 11.20 ± 6.37, and 17.25 ± 3.65-folds were attained, respectively. The expansion reached using L-15 medium in roller bottles was around 10 times higher than that achieved in the static control cultures. To our knowledge, this is the first report of cultures in CO2-free atmosphere to expand cord blood human hematopoietic stem cells and it opens a new branch of possibilities for culturing and clinical applications." @default.
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• W2011714229 date "2011-04-01" @default.
• W2011714229 modified "2023-09-28" @default.
• W2011714229 title "Expansion of Human Hematopoietic Cells from Umbilical Cord Blood Using Roller Bottles in CO₂and CO₂-Free Atmosphere" @default.
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• W2011714229 doi "https://doi.org/10.1089/scd.2010.0236" @default.
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