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- W2011721474 abstract "Light–oxygen–voltage (LOV) domains bind a flavin chromophore to serve as blue light sensors in a wide range of eukaryotic and prokaryotic proteins. LOV domains are associated with a variable effector domain or a separate protein signaling partner to execute a wide variety of functions that include regulation of kinases, generation of anti-sigma factor antagonists, and regulation of circadian clocks. Here we present the crystal structure, photocycle kinetics, association properties, and spectroscopic features of a full-length LOV domain protein from Rhodobacter sphaeroides (RsLOV). RsLOV exhibits N- and C-terminal helical extensions that form an unusual helical bundle at its dimer interface with some resemblance to the helical transducer of sensory rhodopsin II. The blue light-induced conformational changes of RsLOV revealed from a comparison of light- and dark-state crystal structures support a shared signaling mechanism of LOV domain proteins that originates with the light-induced formation of a flavin–cysteinyl photoadduct. Adduct formation disrupts hydrogen bonding in the active site and propagates structural changes through the LOV domain core to the N- and C-terminal extensions. Single-residue variants in the active site and dimer interface of RsLOV alter photoadduct lifetimes and induce structural changes that perturb the oligomeric state. Size exclusion chromatography, multiangle light scattering, small-angle X-ray scattering, and cross-linking studies indicate that RsLOV dimerizes in the dark but, upon light excitation, dissociates into monomers. This light-induced switch in oligomeric state may prove to be useful for engineering molecular associations in controlled cellular settings." @default.
- W2011721474 created "2016-06-24" @default.
- W2011721474 creator A5049500572 @default.
- W2011721474 creator A5071526287 @default.
- W2011721474 creator A5072298588 @default.
- W2011721474 date "2013-01-03" @default.
- W2011721474 modified "2023-10-17" @default.
- W2011721474 title "Light-Induced Subunit Dissociation by a Light–Oxygen–Voltage Domain Photoreceptor from <i>Rhodobacter sphaeroides</i>" @default.
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- W2011721474 doi "https://doi.org/10.1021/bi3015373" @default.
- W2011721474 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3582384" @default.
- W2011721474 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23252338" @default.