FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011749939> ?p ?o ?g. }

• W2011749939 endingPage "215" @default.
• W2011749939 startingPage "205" @default.
• W2011749939 abstract "SUMMARY Macrophages play an important role in the intestinal mucosal immune system. However, they are a poorly defined cell population. We therefore determined their phenotype in normal colonic mucosa. Macrophages were isolated from colonic biopsies and surgical specimens by collagenase digestion. Colonic macrophages were positively sorted by anti-CD33 magnetic beads. Flow cytometric triple fluorescence analysis was applied to study CD14, CD16, CD33, CD44, CD11b, CD11c, CD64, HLA-DR, CD80, CD86 and CD3/CD19 expression. CD33 was evaluated as a positive marker for intestinal macrophages. CD33+ cells isolated from normal colonic mucosa showed co-expression of the established intracellular macrophage marker CD68 in FACS analysis. CD33+ cells were capable of phagocytosis. Isolation of this cell population by magnetic anti-CD33 beads and culture resulted in a 4·2–40-fold increase in IL-1β and 4·5–44-fold increase in tumour necrosis factor-alpha (TNF-α) secretion compared with unsorted lamina propria mononuclear cells (LPMC). Of the CD33+ cells, 90·9 ± 6·9% (mean ± s.d.) were CD44+. However, macrophages from colonic mucosa showed only a low expression of CD14 (10·5 ± 3·8%), CD16 (10·1 ± 3·9%), HLA-DR (27·3 ± 9·2%), CD11b (17·4 ± 6·8%), CD11c (17·8 ± 10·4%). Furthermore, expression of CD80 (9·2 ± 4·2%) and CD86 (15·1 ± 7·3%) was low, suggesting a low ability of normal intestinal macrophages to activate T cells and T cell-mediated immune responses. We conclude that CD33 is useful for the isolation and flow cytometric characterization of colonic macrophages. These cells exhibit a single phenotype in normal mucosa (CD33++, CD44++, CD14−, CD16−, CD11b−, CD11c−, HLA-DRlow, CD80−, CD86−) lacking lipopolysaccharide (LPS) receptor and costimulatory molecules." @default.
• W2011749939 created "2016-06-24" @default.
• W2011749939 creator A5010436037 @default.
• W2011749939 creator A5019876747 @default.
• W2011749939 creator A5020818471 @default.
• W2011749939 creator A5051726567 @default.
• W2011749939 creator A5055423834 @default.
• W2011749939 creator A5058012401 @default.
• W2011749939 creator A5059519023 @default.
• W2011749939 creator A5065186874 @default.
• W2011749939 creator A5065334962 @default.
• W2011749939 date "1998-05-01" @default.
• W2011749939 modified "2023-10-13" @default.
• W2011749939 title "Isolation and phenotypic characterization of colonic macrophages" @default.
• W2011749939 cites W1565321447 @default.
• W2011749939 cites W1600211602 @default.
• W2011749939 cites W1826915530 @default.
• W2011749939 cites W189009910 @default.
• W2011749939 cites W1940535066 @default.
• W2011749939 cites W1970157987 @default.
• W2011749939 cites W1978726442 @default.
• W2011749939 cites W1989210966 @default.
• W2011749939 cites W1996188407 @default.
• W2011749939 cites W2003801599 @default.
• W2011749939 cites W2023702648 @default.
• W2011749939 cites W2029836713 @default.
• W2011749939 cites W2037776391 @default.
• W2011749939 cites W2040838085 @default.
• W2011749939 cites W2045006755 @default.
• W2011749939 cites W2049754653 @default.
• W2011749939 cites W2062870411 @default.
• W2011749939 cites W2065465887 @default.
• W2011749939 cites W2067281865 @default.
• W2011749939 cites W2075298735 @default.
• W2011749939 cites W2076968416 @default.
• W2011749939 cites W2085140635 @default.
• W2011749939 cites W2086406936 @default.
• W2011749939 cites W2104558935 @default.
• W2011749939 cites W2119976037 @default.
• W2011749939 cites W2126489810 @default.
• W2011749939 cites W2138212565 @default.
• W2011749939 cites W2147567659 @default.
• W2011749939 cites W2152414511 @default.
• W2011749939 cites W2165684825 @default.
• W2011749939 cites W2414449585 @default.
• W2011749939 cites W2423286859 @default.
• W2011749939 cites W326932590 @default.
• W2011749939 cites W4302379660 @default.
• W2011749939 doi "https://doi.org/10.1046/j.1365-2249.1998.00557.x" @default.
• W2011749939 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1904962" @default.
• W2011749939 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9649182" @default.
• W2011749939 hasPublicationYear "1998" @default.
• W2011749939 type Work @default.
• W2011749939 sameAs 2011749939 @default.
• W2011749939 citedByCount "169" @default.
• W2011749939 countsByYear W20117499392012 @default.
• W2011749939 countsByYear W20117499392013 @default.
• W2011749939 countsByYear W20117499392014 @default.
• W2011749939 countsByYear W20117499392015 @default.
• W2011749939 countsByYear W20117499392016 @default.
• W2011749939 countsByYear W20117499392017 @default.
• W2011749939 countsByYear W20117499392018 @default.
• W2011749939 countsByYear W20117499392019 @default.
• W2011749939 countsByYear W20117499392020 @default.
• W2011749939 countsByYear W20117499392021 @default.
• W2011749939 countsByYear W20117499392022 @default.
• W2011749939 crossrefType "journal-article" @default.
• W2011749939 hasAuthorship W2011749939A5010436037 @default.
• W2011749939 hasAuthorship W2011749939A5019876747 @default.
• W2011749939 hasAuthorship W2011749939A5020818471 @default.
• W2011749939 hasAuthorship W2011749939A5051726567 @default.
• W2011749939 hasAuthorship W2011749939A5055423834 @default.
• W2011749939 hasAuthorship W2011749939A5058012401 @default.
• W2011749939 hasAuthorship W2011749939A5059519023 @default.
• W2011749939 hasAuthorship W2011749939A5065186874 @default.
• W2011749939 hasAuthorship W2011749939A5065334962 @default.
• W2011749939 hasBestOaLocation W20117499391 @default.
• W2011749939 hasConcept C126322002 @default.
• W2011749939 hasConcept C153911025 @default.
• W2011749939 hasConcept C154317977 @default.
• W2011749939 hasConcept C202751555 @default.
• W2011749939 hasConcept C203014093 @default.
• W2011749939 hasConcept C2776090121 @default.
• W2011749939 hasConcept C2777310793 @default.
• W2011749939 hasConcept C2778297628 @default.
• W2011749939 hasConcept C2781101188 @default.
• W2011749939 hasConcept C2908647359 @default.
• W2011749939 hasConcept C3019368612 @default.
• W2011749939 hasConcept C39347974 @default.
• W2011749939 hasConcept C529295009 @default.
• W2011749939 hasConcept C54355233 @default.
• W2011749939 hasConcept C55493867 @default.
• W2011749939 hasConcept C71924100 @default.
• W2011749939 hasConcept C86803240 @default.
• W2011749939 hasConcept C8891405 @default.
• W2011749939 hasConcept C99454951 @default.
• W2011749939 hasConceptScore W2011749939C126322002 @default.
• W2011749939 hasConceptScore W2011749939C153911025 @default.

• next