Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011749940> ?p ?o ?g. }
- W2011749940 endingPage "5432" @default.
- W2011749940 startingPage "5423" @default.
- W2011749940 abstract "ABSTRACT In most streptococci, glucose is transported by the phosphoenolpyruvate (PEP):glucose/mannose phosphotransferase system (PTS) via HPr and IIAB Man , two proteins involved in regulatory mechanisms. While most strains of Streptococcus thermophilus do not or poorly metabolize glucose, compelling evidence suggests that S. thermophilus possesses the genes that encode the glucose/mannose general and specific PTS proteins. The purposes of this study were to determine (i) whether these PTS genes are expressed, (ii) whether the PTS proteins encoded by these genes are able to transfer a phosphate group from PEP to glucose/mannose PTS substrates, and (iii) whether these proteins catalyze sugar transport. The pts operon is made up of the genes encoding HPr ( ptsH ) and enzyme I (EI) ( ptsI ), which are transcribed into a 0.6-kb ptsH mRNA and a 2.3-kb ptsHI mRNA. The specific glucose/mannose PTS proteins, IIAB Man , IIC Man , IID Man , and the ManO protein, are encoded by manL , manM , manN , and manO , respectively, which make up the man operon. The man operon is transcribed into a single 3.5-kb mRNA. To assess the phosphotransfer competence of these PTS proteins, in vitro PEP-dependent phosphorylation experiments were conducted with purified HPr, EI, and IIAB Man as well as membrane fragments containing IIC Man and IID Man . These PTS components efficiently transferred a phosphate group from PEP to glucose, mannose, 2-deoxyglucose, and (to a lesser extent) fructose, which are common streptococcal glucose/mannose PTS substrates. Whole cells were unable to catalyze the uptake of mannose and 2-deoxyglucose, demonstrating the inability of the S. thermophilus PTS proteins to operate as a proficient transport system. This inability to transport mannose and 2-deoxyglucose may be due to a defective IIC domain. We propose that in S. thermophilus , the general and specific glucose/mannose PTS proteins are not involved in glucose transport but might have regulatory functions associated with the phosphotransfer properties of HPr and IIAB Man ." @default.
- W2011749940 created "2016-06-24" @default.
- W2011749940 creator A5012234198 @default.
- W2011749940 creator A5021652467 @default.
- W2011749940 creator A5035233924 @default.
- W2011749940 creator A5079257651 @default.
- W2011749940 date "2003-09-01" @default.
- W2011749940 modified "2023-10-14" @default.
- W2011749940 title "Genetic and Biochemical Characterization of the Phosphoenolpyruvate:Glucose/Mannose Phosphotransferase System of <i>Streptococcus thermophilus</i>" @default.
- W2011749940 cites W1491979783 @default.
- W2011749940 cites W1497575646 @default.
- W2011749940 cites W1498737617 @default.
- W2011749940 cites W1546793327 @default.
- W2011749940 cites W1575545802 @default.
- W2011749940 cites W1858328737 @default.
- W2011749940 cites W1940385821 @default.
- W2011749940 cites W1951846066 @default.
- W2011749940 cites W1963906950 @default.
- W2011749940 cites W1972630690 @default.
- W2011749940 cites W1974647232 @default.
- W2011749940 cites W1986002586 @default.
- W2011749940 cites W1995295185 @default.
- W2011749940 cites W2003282477 @default.
- W2011749940 cites W2004138468 @default.
- W2011749940 cites W2009310436 @default.
- W2011749940 cites W2009816989 @default.
- W2011749940 cites W2015522163 @default.
- W2011749940 cites W2026129201 @default.
- W2011749940 cites W2036502925 @default.
- W2011749940 cites W2037474706 @default.
- W2011749940 cites W2038797014 @default.
- W2011749940 cites W2039702459 @default.
- W2011749940 cites W2046737461 @default.
- W2011749940 cites W2050311665 @default.
- W2011749940 cites W2058911298 @default.
- W2011749940 cites W2060461460 @default.
- W2011749940 cites W2062482981 @default.
- W2011749940 cites W2063800359 @default.
- W2011749940 cites W2071593518 @default.
- W2011749940 cites W2074301908 @default.
- W2011749940 cites W2074660125 @default.
- W2011749940 cites W2085154839 @default.
- W2011749940 cites W2087574062 @default.
- W2011749940 cites W2095025323 @default.
- W2011749940 cites W2100837269 @default.
- W2011749940 cites W2110973292 @default.
- W2011749940 cites W2127321248 @default.
- W2011749940 cites W2129282478 @default.
- W2011749940 cites W2130394726 @default.
- W2011749940 cites W2132417094 @default.
- W2011749940 cites W2139325759 @default.
- W2011749940 cites W2139495241 @default.
- W2011749940 cites W2141589508 @default.
- W2011749940 cites W2144516494 @default.
- W2011749940 cites W2146675943 @default.
- W2011749940 cites W2160038593 @default.
- W2011749940 cites W2160837490 @default.
- W2011749940 cites W2167742785 @default.
- W2011749940 cites W2169934605 @default.
- W2011749940 cites W989525154 @default.
- W2011749940 doi "https://doi.org/10.1128/aem.69.9.5423-5432.2003" @default.
- W2011749940 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/194979" @default.
- W2011749940 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12957931" @default.
- W2011749940 hasPublicationYear "2003" @default.
- W2011749940 type Work @default.
- W2011749940 sameAs 2011749940 @default.
- W2011749940 citedByCount "33" @default.
- W2011749940 countsByYear W20117499402012 @default.
- W2011749940 countsByYear W20117499402013 @default.
- W2011749940 countsByYear W20117499402015 @default.
- W2011749940 countsByYear W20117499402016 @default.
- W2011749940 countsByYear W20117499402018 @default.
- W2011749940 countsByYear W20117499402021 @default.
- W2011749940 countsByYear W20117499402022 @default.
- W2011749940 crossrefType "journal-article" @default.
- W2011749940 hasAuthorship W2011749940A5012234198 @default.
- W2011749940 hasAuthorship W2011749940A5021652467 @default.
- W2011749940 hasAuthorship W2011749940A5035233924 @default.
- W2011749940 hasAuthorship W2011749940A5079257651 @default.
- W2011749940 hasBestOaLocation W20117499402 @default.
- W2011749940 hasConcept C100544194 @default.
- W2011749940 hasConcept C104317684 @default.
- W2011749940 hasConcept C143937172 @default.
- W2011749940 hasConcept C153911025 @default.
- W2011749940 hasConcept C181199279 @default.
- W2011749940 hasConcept C203075996 @default.
- W2011749940 hasConcept C2775887612 @default.
- W2011749940 hasConcept C2777276588 @default.
- W2011749940 hasConcept C2777504003 @default.
- W2011749940 hasConcept C2780206646 @default.
- W2011749940 hasConcept C45656491 @default.
- W2011749940 hasConcept C547475151 @default.
- W2011749940 hasConcept C55493867 @default.
- W2011749940 hasConcept C86803240 @default.
- W2011749940 hasConceptScore W2011749940C100544194 @default.
- W2011749940 hasConceptScore W2011749940C104317684 @default.
- W2011749940 hasConceptScore W2011749940C143937172 @default.
- W2011749940 hasConceptScore W2011749940C153911025 @default.