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• W2011750206 abstract "Metallothioneins (MT) are ubiquitous low molecular weight metal binding proteins that may act as antioxidants. In the present study, the cloned human MT-III coding region was permanently transfected into GM00637 cells in order to investigate the antioxidative effects of this brain-specific MT isoform. GM00637/MT-III cells overexpressed MT-III mRNA versus pcDNA3 plasmid-transfected control cells (GM00637). When challenged with H(2)O(2), the GM00637/MT-III cells displayed significantly more resistance than the GM00637 cells, as determined by cell cytotoxicity, lactate dehydrogenase leakage, and lipid peroxidation. In addition, the GM00637/MT-III cells were highly protected from the H(2)O(2)-induced production of reactive oxygen species and DNA damage. These results directly support the antioxidative role of MT-III, and demonstrate MT-III can scavenge free oxygen radicals and protect cells from oxidative stress." @default.
• W2011750206 created "2016-06-24" @default.
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• W2011750206 date "2002-05-30" @default.
• W2011750206 modified "2023-09-25" @default.
• W2011750206 title "Overexpression of human metallothionein-III prevents hydrogen peroxide-induced oxidative stress in human fibroblasts" @default.
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• W2011750206 doi "https://doi.org/10.1016/s0014-5793(02)02870-3" @default.
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