FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011759126> ?p ?o ?g. }

• W2011759126 endingPage "4980" @default.
• W2011759126 startingPage "4974" @default.
• W2011759126 abstract "Lentil lectin (LcH) and pea lectin (PSA) belong to the class of D-glucose/D-mannose binding lectins and resemble concanavalin A (Con A) closely in physicochemical, structural, and biological properties. LcH and PSA, like Con A, are Ca2+-Mn2+ metalloproteins that require the metal ions for their saccharide binding and biological activities. Studies of the relationship between the metal ions binding and saccharide binding activity in LcH and PSA have been difficult due to the problem of metal ion replacement in these proteins. We now report a method of metal ion replacement in both lectins that allows substitution of the Mn2+ in the native proteins with a variety of transition metal ions, as well as substitution of the Ca2+ with Cd2+ in a particular complex. The following metal ion derivatives of both LcH and PSA have been prepared: Ca2+-Zn2+, Ca2+-Co2+, Ca2+-Ni2+, and Cd2+-Cd2+. All of these derivatives are as active as the native lectins, as demonstrated by precipitation with specific polysaccharides, saccharide inhibition of precipitation, and hemagglutination assays. The yields of these derivatives are good (generally greater than 70%), and the degree of metal ion incorporation is high (generally greater than 90%). The method of preparation is quite different from that for metal ion substitution in Con A, which proceeds via the apoprotein. In contrast, the apoproteins of LcH and PSA are unstable, aggregate above pH 4.0, and cannot be remetallized once formed.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W2011759126 created "2016-06-24" @default.
• W2011759126 creator A5022650078 @default.
• W2011759126 creator A5028326396 @default.
• W2011759126 creator A5039138618 @default.
• W2011759126 creator A5074044896 @default.
• W2011759126 date "1985-09-01" @default.
• W2011759126 modified "2023-09-26" @default.
• W2011759126 title "Preparation and properties of metal derivatives of the lentil and pea lectins" @default.
• W2011759126 cites W1530723302 @default.
• W2011759126 cites W1533411461 @default.
• W2011759126 cites W1560923897 @default.
• W2011759126 cites W1563862675 @default.
• W2011759126 cites W1584587121 @default.
• W2011759126 cites W1591050105 @default.
• W2011759126 cites W1758850846 @default.
• W2011759126 cites W1864838378 @default.
• W2011759126 cites W1906831495 @default.
• W2011759126 cites W1994974848 @default.
• W2011759126 cites W1995826320 @default.
• W2011759126 cites W1996284888 @default.
• W2011759126 cites W1996794766 @default.
• W2011759126 cites W2015876721 @default.
• W2011759126 cites W2024772889 @default.
• W2011759126 cites W2025095806 @default.
• W2011759126 cites W2026562921 @default.
• W2011759126 cites W2034894954 @default.
• W2011759126 cites W2044033134 @default.
• W2011759126 cites W2050510945 @default.
• W2011759126 cites W2059665556 @default.
• W2011759126 cites W2065916070 @default.
• W2011759126 cites W2069702867 @default.
• W2011759126 cites W2071974508 @default.
• W2011759126 cites W2075762780 @default.
• W2011759126 cites W2084101844 @default.
• W2011759126 cites W2088087860 @default.
• W2011759126 cites W2091097108 @default.
• W2011759126 cites W215057969 @default.
• W2011759126 cites W2154009660 @default.
• W2011759126 cites W2215282776 @default.
• W2011759126 cites W2267312560 @default.
• W2011759126 cites W2277298148 @default.
• W2011759126 cites W2401320867 @default.
• W2011759126 doi "https://doi.org/10.1021/bi00340a002" @default.
• W2011759126 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4074668" @default.
• W2011759126 hasPublicationYear "1985" @default.
• W2011759126 type Work @default.
• W2011759126 sameAs 2011759126 @default.
• W2011759126 citedByCount "17" @default.
• W2011759126 countsByYear W20117591262012 @default.
• W2011759126 countsByYear W20117591262015 @default.
• W2011759126 countsByYear W20117591262023 @default.
• W2011759126 crossrefType "journal-article" @default.
• W2011759126 hasAuthorship W2011759126A5022650078 @default.
• W2011759126 hasAuthorship W2011759126A5028326396 @default.
• W2011759126 hasAuthorship W2011759126A5039138618 @default.
• W2011759126 hasAuthorship W2011759126A5074044896 @default.
• W2011759126 hasConcept C107054158 @default.
• W2011759126 hasConcept C121332964 @default.
• W2011759126 hasConcept C153294291 @default.
• W2011759126 hasConcept C178790620 @default.
• W2011759126 hasConcept C185592680 @default.
• W2011759126 hasConcept C19869320 @default.
• W2011759126 hasConcept C199164860 @default.
• W2011759126 hasConcept C202751555 @default.
• W2011759126 hasConcept C2780758401 @default.
• W2011759126 hasConcept C544153396 @default.
• W2011759126 hasConcept C55493867 @default.
• W2011759126 hasConcept C71240020 @default.
• W2011759126 hasConceptScore W2011759126C107054158 @default.
• W2011759126 hasConceptScore W2011759126C121332964 @default.
• W2011759126 hasConceptScore W2011759126C153294291 @default.
• W2011759126 hasConceptScore W2011759126C178790620 @default.
• W2011759126 hasConceptScore W2011759126C185592680 @default.
• W2011759126 hasConceptScore W2011759126C19869320 @default.
• W2011759126 hasConceptScore W2011759126C199164860 @default.
• W2011759126 hasConceptScore W2011759126C202751555 @default.
• W2011759126 hasConceptScore W2011759126C2780758401 @default.
• W2011759126 hasConceptScore W2011759126C544153396 @default.
• W2011759126 hasConceptScore W2011759126C55493867 @default.
• W2011759126 hasConceptScore W2011759126C71240020 @default.
• W2011759126 hasIssue "19" @default.
• W2011759126 hasLocation W20117591261 @default.
• W2011759126 hasLocation W20117591262 @default.
• W2011759126 hasOpenAccess W2011759126 @default.
• W2011759126 hasPrimaryLocation W20117591261 @default.
• W2011759126 hasRelatedWork W1975356509 @default.
• W2011759126 hasRelatedWork W1978994833 @default.
• W2011759126 hasRelatedWork W2009215244 @default.
• W2011759126 hasRelatedWork W2029302581 @default.
• W2011759126 hasRelatedWork W2051275938 @default.
• W2011759126 hasRelatedWork W2185831673 @default.
• W2011759126 hasRelatedWork W2272161374 @default.
• W2011759126 hasRelatedWork W2598004097 @default.
• W2011759126 hasRelatedWork W4320916897 @default.
• W2011759126 hasRelatedWork W54689422 @default.
• W2011759126 hasVolume "24" @default.
• W2011759126 isParatext "false" @default.

• next