FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011760688> ?p ?o ?g. }

• W2011760688 endingPage "140" @default.
• W2011760688 startingPage "132" @default.
• W2011760688 abstract "Recombinant adenosylcobalamin-dependent diol dehydratase ofKlebsiella oxytocaoverexpressed inEscherichia coliwas purified to homogeneity. The enzyme has a low solubility and was extracted from the crude membrane fraction with 1% Brij 35 in a high recovery. Subsequent chromatography on DEAE–cellulose resulted in 4.9-fold purification of the enzyme in an overall yield of 65%. The enzyme thus obtained showed specific activity comparable to that of the wild-type enzyme ofK. oxytoca.The apparent molecular weight determined by nondenaturing gel electrophoresis on a gradient gel was 220,000. The enzyme consists of equimolar amounts of the three subunits with apparentMrof 60,000 (α), 30,000 (β), and 19,000 (γ). Therefore, the subunit structure of the enzyme is most likely α2β2γ2. The recombinant enzyme was also separated into components F and S upon DEAE–cellulose chromatography in the absence of substrate. Components F and S were identified as the β subunit and α2γ2complex, respectively. ApparentKmfor adenosylcobalamin, 1,2-propanediol, glycerol, and 1,2-ethanediol were 0.83 μm, 0.08 mm, 0.73 mm, and 0.56 mm, respectively. The three genes encoding the subunits of diol dehydratase were overexpressed individually or in various combinations inEscherichia coli.The α and γ subunits mutually required each other for correct folding forming the soluble, active α2γ2complex (component S). Expression of the β subunit in a soluble, active form (component F) was promoted by coexpression with both the α and γ subunits, probably by coexistence with component S. These lines of evidence indicate that each subunit mutually affects the folding of the others in this heterooligomer enzyme." @default.
• W2011760688 created "2016-06-24" @default.
• W2011760688 creator A5001368981 @default.
• W2011760688 creator A5014018538 @default.
• W2011760688 creator A5037269319 @default.
• W2011760688 creator A5072959750 @default.
• W2011760688 creator A5077518647 @default.
• W2011760688 creator A5079245878 @default.
• W2011760688 date "1997-11-01" @default.
• W2011760688 modified "2023-10-03" @default.
• W2011760688 title "Heterologous Expression, Purification, and Properties of Diol Dehydratase, an Adenosylcobalamin-Dependent Enzyme ofKlebsiella oxytoca" @default.
• W2011760688 cites W1480773097 @default.
• W2011760688 cites W1493485729 @default.
• W2011760688 cites W1551238362 @default.
• W2011760688 cites W1553501991 @default.
• W2011760688 cites W1555021163 @default.
• W2011760688 cites W1571527712 @default.
• W2011760688 cites W1583668498 @default.
• W2011760688 cites W1775749144 @default.
• W2011760688 cites W1957986694 @default.
• W2011760688 cites W1968456189 @default.
• W2011760688 cites W1983277269 @default.
• W2011760688 cites W1986264642 @default.
• W2011760688 cites W1988099644 @default.
• W2011760688 cites W1995013470 @default.
• W2011760688 cites W1996817025 @default.
• W2011760688 cites W2004186744 @default.
• W2011760688 cites W2014503751 @default.
• W2011760688 cites W2025501776 @default.
• W2011760688 cites W2040857161 @default.
• W2011760688 cites W2042930193 @default.
• W2011760688 cites W2060490165 @default.
• W2011760688 cites W2070430204 @default.
• W2011760688 cites W2079330271 @default.
• W2011760688 cites W2079867747 @default.
• W2011760688 cites W2081243527 @default.
• W2011760688 cites W2100837269 @default.
• W2011760688 cites W2111301002 @default.
• W2011760688 cites W2120695725 @default.
• W2011760688 cites W2232309423 @default.
• W2011760688 cites W2299511190 @default.
• W2011760688 cites W3206291250 @default.
• W2011760688 doi "https://doi.org/10.1006/abbi.1997.0325" @default.
• W2011760688 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9344474" @default.
• W2011760688 hasPublicationYear "1997" @default.
• W2011760688 type Work @default.
• W2011760688 sameAs 2011760688 @default.
• W2011760688 citedByCount "43" @default.
• W2011760688 countsByYear W20117606882012 @default.
• W2011760688 countsByYear W20117606882013 @default.
• W2011760688 countsByYear W20117606882014 @default.
• W2011760688 countsByYear W20117606882015 @default.
• W2011760688 countsByYear W20117606882017 @default.
• W2011760688 countsByYear W20117606882018 @default.
• W2011760688 countsByYear W20117606882021 @default.
• W2011760688 countsByYear W20117606882022 @default.
• W2011760688 crossrefType "journal-article" @default.
• W2011760688 hasAuthorship W2011760688A5001368981 @default.
• W2011760688 hasAuthorship W2011760688A5014018538 @default.
• W2011760688 hasAuthorship W2011760688A5037269319 @default.
• W2011760688 hasAuthorship W2011760688A5072959750 @default.
• W2011760688 hasAuthorship W2011760688A5077518647 @default.
• W2011760688 hasAuthorship W2011760688A5079245878 @default.
• W2011760688 hasConcept C104292427 @default.
• W2011760688 hasConcept C104317684 @default.
• W2011760688 hasConcept C179247698 @default.
• W2011760688 hasConcept C181199279 @default.
• W2011760688 hasConcept C185592680 @default.
• W2011760688 hasConcept C197957613 @default.
• W2011760688 hasConcept C198684833 @default.
• W2011760688 hasConcept C2778979354 @default.
• W2011760688 hasConcept C2781374117 @default.
• W2011760688 hasConcept C40767141 @default.
• W2011760688 hasConcept C43617362 @default.
• W2011760688 hasConcept C55493867 @default.
• W2011760688 hasConceptScore W2011760688C104292427 @default.
• W2011760688 hasConceptScore W2011760688C104317684 @default.
• W2011760688 hasConceptScore W2011760688C179247698 @default.
• W2011760688 hasConceptScore W2011760688C181199279 @default.
• W2011760688 hasConceptScore W2011760688C185592680 @default.
• W2011760688 hasConceptScore W2011760688C197957613 @default.
• W2011760688 hasConceptScore W2011760688C198684833 @default.
• W2011760688 hasConceptScore W2011760688C2778979354 @default.
• W2011760688 hasConceptScore W2011760688C2781374117 @default.
• W2011760688 hasConceptScore W2011760688C40767141 @default.
• W2011760688 hasConceptScore W2011760688C43617362 @default.
• W2011760688 hasConceptScore W2011760688C55493867 @default.
• W2011760688 hasFunder F4320320912 @default.
• W2011760688 hasFunder F4320325524 @default.
• W2011760688 hasFunder F4320325525 @default.
• W2011760688 hasFunder F4320334764 @default.
• W2011760688 hasIssue "1" @default.
• W2011760688 hasLocation W20117606881 @default.
• W2011760688 hasLocation W20117606882 @default.
• W2011760688 hasOpenAccess W2011760688 @default.
• W2011760688 hasPrimaryLocation W20117606881 @default.
• W2011760688 hasRelatedWork W1850437025 @default.
• W2011760688 hasRelatedWork W1976314208 @default.

• next