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• W2011831809 endingPage "300" @default.
• W2011831809 startingPage "287" @default.
• W2011831809 abstract "Nanodiscs are self-assembled discoidal phospholipid bilayers surrounded and stabilized by membrane scaffold proteins (MSPs), that have become a powerful and promising tool for the study of membrane proteins. Even though their reconstitution is highly regulated by the type of MSP and phospholipid input, a biophysical characterization leading to the determination of the stoichiometry of MSP, lipid and membrane protein is essential. This is important for biological studies, as the oligomeric state of membrane proteins often correlates with their functional activity. Typically combinations of several methods are applied using, for example, modified samples that incorporate fluorescent labels, along with procedures that result in nanodisc disassembly and lipid dissolution. To obtain a comprehensive understanding of the native properties of nanodiscs, modification-free analysis methods are required. In this work we provide a strategy, using a combination of dynamic light scattering and analytical ultracentrifugation, for the biophysical characterization of unmodified nanodiscs. In this manner we characterize the nanodisc preparation in terms of its overall polydispersity and characterize the hydrodynamically resolved nanodisc of interest in terms of its sedimentation coefficient, Stokes’ radius and overall protein and lipid stoichiometry. Functional and biological applications are also discussed for the study of the membrane protein embedded in nanodiscs under defined experimental conditions." @default.
• W2011831809 created "2016-06-24" @default.
• W2011831809 creator A5012620108 @default.
• W2011831809 creator A5039383940 @default.
• W2011831809 creator A5074374757 @default.
• W2011831809 date "2013-03-01" @default.
• W2011831809 modified "2023-10-12" @default.
• W2011831809 title "Biophysical characterization of membrane proteins in nanodiscs" @default.
• W2011831809 cites W1503336891 @default.
• W2011831809 cites W1556888008 @default.
• W2011831809 cites W1565197201 @default.
• W2011831809 cites W1598629441 @default.
• W2011831809 cites W1607957872 @default.
• W2011831809 cites W1753435687 @default.
• W2011831809 cites W1879047861 @default.
• W2011831809 cites W1970861588 @default.
• W2011831809 cites W1973090022 @default.
• W2011831809 cites W1974700900 @default.
• W2011831809 cites W1975317507 @default.
• W2011831809 cites W1977700439 @default.
• W2011831809 cites W1977839039 @default.
• W2011831809 cites W1981530905 @default.
• W2011831809 cites W1984981562 @default.
• W2011831809 cites W1990322965 @default.
• W2011831809 cites W1995272207 @default.
• W2011831809 cites W1997355092 @default.
• W2011831809 cites W1997394451 @default.
• W2011831809 cites W2005574282 @default.
• W2011831809 cites W2007002347 @default.
• W2011831809 cites W2016517180 @default.
• W2011831809 cites W2021162754 @default.
• W2011831809 cites W2024943051 @default.
• W2011831809 cites W2026240692 @default.
• W2011831809 cites W2033914586 @default.
• W2011831809 cites W2035048137 @default.
• W2011831809 cites W2035852278 @default.
• W2011831809 cites W2036751098 @default.
• W2011831809 cites W2040714543 @default.
• W2011831809 cites W2040795711 @default.
• W2011831809 cites W2041088894 @default.
• W2011831809 cites W2041211150 @default.
• W2011831809 cites W2044948606 @default.
• W2011831809 cites W2046491379 @default.
• W2011831809 cites W2052311523 @default.
• W2011831809 cites W2053802188 @default.
• W2011831809 cites W2054342311 @default.
• W2011831809 cites W2054479516 @default.
• W2011831809 cites W2054767423 @default.
• W2011831809 cites W2055218251 @default.
• W2011831809 cites W2057148477 @default.
• W2011831809 cites W2058268622 @default.
• W2011831809 cites W2059160047 @default.
• W2011831809 cites W2062789181 @default.
• W2011831809 cites W2063080724 @default.
• W2011831809 cites W2063868052 @default.
• W2011831809 cites W2064730449 @default.
• W2011831809 cites W2064910975 @default.
• W2011831809 cites W2065915076 @default.
• W2011831809 cites W2069520354 @default.
• W2011831809 cites W2070020792 @default.
• W2011831809 cites W2074370114 @default.
• W2011831809 cites W2076141179 @default.
• W2011831809 cites W2076166680 @default.
• W2011831809 cites W2078034467 @default.
• W2011831809 cites W2078108096 @default.
• W2011831809 cites W2078149957 @default.
• W2011831809 cites W2079619903 @default.
• W2011831809 cites W2082497364 @default.
• W2011831809 cites W2083576886 @default.
• W2011831809 cites W2085563968 @default.
• W2011831809 cites W2086117323 @default.
• W2011831809 cites W2086793204 @default.
• W2011831809 cites W2089468762 @default.
• W2011831809 cites W2089617519 @default.
• W2011831809 cites W2090187175 @default.
• W2011831809 cites W2090261172 @default.
• W2011831809 cites W2092832994 @default.
• W2011831809 cites W2096601563 @default.
• W2011831809 cites W2099134954 @default.
• W2011831809 cites W2103912693 @default.
• W2011831809 cites W2111125193 @default.
• W2011831809 cites W2113183595 @default.
• W2011831809 cites W2123888304 @default.
• W2011831809 cites W2125872855 @default.
• W2011831809 cites W2129243467 @default.
• W2011831809 cites W2129497784 @default.
• W2011831809 cites W2140770667 @default.
• W2011831809 cites W2149378523 @default.
• W2011831809 cites W2151929256 @default.
• W2011831809 cites W2152208386 @default.
• W2011831809 cites W2154351186 @default.
• W2011831809 cites W2155909615 @default.
• W2011831809 cites W2163449711 @default.
• W2011831809 cites W2164993892 @default.
• W2011831809 cites W2171038998 @default.
• W2011831809 cites W2171621506 @default.
• W2011831809 cites W2171687207 @default.
• W2011831809 cites W2179386188 @default.

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