FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011833557> ?p ?o ?g. }

• W2011833557 endingPage "510" @default.
• W2011833557 startingPage "496" @default.
• W2011833557 abstract "Here we present the in vitro selection of a novel ribozyme specific for Zn2+-dependent catalysis on hydrolysis of a phosphorothiolate thiolester bond. The ribozyme, called the TW17 ribozyme, was evolved and selected from an artificial RNA pool covalently linked to a biotin-containing substrate through the phosphorothiolate thiolester bond. The secondary structure for the evolved ribozyme consisted of three major helices and three loops. Biochemical and chemical studies of ribozyme-catalyzed reaction products provided evidence that the ribozyme specifically catalyzes hydrolysis of the phosphorothiolate thiolester linkage. A successful ribozyme construct with active catalysis in trans further supported the determined ribozyme structure and indicated the potential of the ribozyme for multiple-substrate turnover. The ribozyme also requires Zn2+ and Mg2+ for maximal catalysis. The TW17 ribozyme, in the presence of Zn2+ and Mg2+, conferred a rate enhancement of at least 5 orders of magnitude when compared to the estimated rate of the uncatalyzed reaction. The ribozyme completely lost catalytic activity in the absence of Zn2+, like Zn2+-dependent protein hydrolases. The discovery and characterization of the TW17 ribozyme suggest additional roles for Zn2+ in ribozyme catalysts." @default.
• W2011833557 created "2016-06-24" @default.
• W2011833557 creator A5011686559 @default.
• W2011833557 creator A5017415084 @default.
• W2011833557 creator A5033780365 @default.
• W2011833557 creator A5036614191 @default.
• W2011833557 creator A5048036284 @default.
• W2011833557 creator A5059149914 @default.
• W2011833557 creator A5063800558 @default.
• W2011833557 creator A5083955479 @default.
• W2011833557 creator A5091688671 @default.
• W2011833557 date "2011-12-28" @default.
• W2011833557 modified "2023-10-14" @default.
• W2011833557 title "In Vitro Selection and Characterization of a Novel Zn(II)-Dependent Phosphorothiolate Thiolesterase Ribozyme" @default.
• W2011833557 cites W1633944576 @default.
• W2011833557 cites W1951188479 @default.
• W2011833557 cites W1966869754 @default.
• W2011833557 cites W1968282235 @default.
• W2011833557 cites W1981147068 @default.
• W2011833557 cites W1982554906 @default.
• W2011833557 cites W1984319638 @default.
• W2011833557 cites W1984852608 @default.
• W2011833557 cites W1996439321 @default.
• W2011833557 cites W2004945910 @default.
• W2011833557 cites W2021333801 @default.
• W2011833557 cites W2023639964 @default.
• W2011833557 cites W2029181479 @default.
• W2011833557 cites W2040658284 @default.
• W2011833557 cites W2048266374 @default.
• W2011833557 cites W2050110866 @default.
• W2011833557 cites W2051997395 @default.
• W2011833557 cites W2052044977 @default.
• W2011833557 cites W2066928237 @default.
• W2011833557 cites W2073775872 @default.
• W2011833557 cites W2085158278 @default.
• W2011833557 cites W2091535033 @default.
• W2011833557 cites W2095239103 @default.
• W2011833557 cites W2097335018 @default.
• W2011833557 cites W2104245738 @default.
• W2011833557 cites W2119342504 @default.
• W2011833557 cites W2120434145 @default.
• W2011833557 cites W2128934600 @default.
• W2011833557 cites W2134988785 @default.
• W2011833557 cites W2136830750 @default.
• W2011833557 cites W2140956967 @default.
• W2011833557 cites W2145144268 @default.
• W2011833557 cites W2146837193 @default.
• W2011833557 cites W2149005864 @default.
• W2011833557 cites W2156833843 @default.
• W2011833557 cites W2160305643 @default.
• W2011833557 cites W2418628612 @default.
• W2011833557 doi "https://doi.org/10.1021/bi201585d" @default.
• W2011833557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22175782" @default.
• W2011833557 hasPublicationYear "2011" @default.
• W2011833557 type Work @default.
• W2011833557 sameAs 2011833557 @default.
• W2011833557 citedByCount "8" @default.
• W2011833557 countsByYear W20118335572012 @default.
• W2011833557 countsByYear W20118335572013 @default.
• W2011833557 countsByYear W20118335572014 @default.
• W2011833557 countsByYear W20118335572016 @default.
• W2011833557 countsByYear W20118335572018 @default.
• W2011833557 countsByYear W20118335572020 @default.
• W2011833557 crossrefType "journal-article" @default.
• W2011833557 hasAuthorship W2011833557A5011686559 @default.
• W2011833557 hasAuthorship W2011833557A5017415084 @default.
• W2011833557 hasAuthorship W2011833557A5033780365 @default.
• W2011833557 hasAuthorship W2011833557A5036614191 @default.
• W2011833557 hasAuthorship W2011833557A5048036284 @default.
• W2011833557 hasAuthorship W2011833557A5059149914 @default.
• W2011833557 hasAuthorship W2011833557A5063800558 @default.
• W2011833557 hasAuthorship W2011833557A5083955479 @default.
• W2011833557 hasAuthorship W2011833557A5091688671 @default.
• W2011833557 hasConcept C104317684 @default.
• W2011833557 hasConcept C107745601 @default.
• W2011833557 hasConcept C1342335 @default.
• W2011833557 hasConcept C185592680 @default.
• W2011833557 hasConcept C30951146 @default.
• W2011833557 hasConcept C55493867 @default.
• W2011833557 hasConcept C66144319 @default.
• W2011833557 hasConcept C67705224 @default.
• W2011833557 hasConcept C71240020 @default.
• W2011833557 hasConcept C76346623 @default.
• W2011833557 hasConceptScore W2011833557C104317684 @default.
• W2011833557 hasConceptScore W2011833557C107745601 @default.
• W2011833557 hasConceptScore W2011833557C1342335 @default.
• W2011833557 hasConceptScore W2011833557C185592680 @default.
• W2011833557 hasConceptScore W2011833557C30951146 @default.
• W2011833557 hasConceptScore W2011833557C55493867 @default.
• W2011833557 hasConceptScore W2011833557C66144319 @default.
• W2011833557 hasConceptScore W2011833557C67705224 @default.
• W2011833557 hasConceptScore W2011833557C71240020 @default.
• W2011833557 hasConceptScore W2011833557C76346623 @default.
• W2011833557 hasIssue "1" @default.
• W2011833557 hasLocation W20118335571 @default.
• W2011833557 hasLocation W20118335572 @default.
• W2011833557 hasOpenAccess W2011833557 @default.
• W2011833557 hasPrimaryLocation W20118335571 @default.

• next