FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011840549> ?p ?o ?g. }

• W2011840549 endingPage "115" @default.
• W2011840549 startingPage "109" @default.
• W2011840549 abstract "Transgenic Citrus sinensis (L.) Osb. cv. Hamlin plants expressing the hrpN gene were obtained by Agrobacterium tumefaciens (Smith and Towns) Conn-mediated transformation. hrpN encodes a harpin protein, which elicits the hypersensitive response and systemic acquired resistance in plants. The gene construct consisted of gst1, a pathogen-inducible promoter, a signal peptide for protein secretion to the apoplast, the selection genes nptII or aacC1 and the Nos terminator. The function of gst1 in citrus was evaluated in transgenic C. sinensis cv. Valencia harboring the reporter gene uidA (gus) driven by this promoter. Histochemical analysis for gus revealed that gst1 is activated in citrus leaves by both wounding and inoculation with Xanthomonas axonopodis Starr and Garces pv. citri (Hasse) Vauterin et al. Genetic transformation was confirmed by Southern blot hybridization in eight cv. Hamlin acclimatized plants. RT-PCR confirmed hrpN gene expression in seven cv. Hamlin transgenic lines before pathogen inoculation. Some hrpN transgenic lines showed severe leaf curling and abnormal growth. Six hrpN transgenic lines were propagated and evaluated for susceptibility to X. axonopodis pv. citri. RT-PCR confirmed gene expression in all six hrpN transgenic lines after pathogen inoculation. Several of the hrpN transgenic lines showed reduction in susceptibility to citrus canker as compared with non-transgenic plants. One hrpN transgenic line exhibited normal vegetative development and displayed very high resistance to the pathogen, estimated as up to 79% reduction in disease severity. This is the first report of genetic transformation of citrus using a pathogen-inducible promoter and the hrpN gene. Further evaluations of the transgenic plants under field conditions are planned. Nevertheless, the evidence to date suggests that the hrpN gene reduces the susceptibility of citrus plants to the canker disease." @default.
• W2011840549 created "2016-06-24" @default.
• W2011840549 creator A5006002742 @default.
• W2011840549 creator A5008896828 @default.
• W2011840549 creator A5017792944 @default.
• W2011840549 creator A5040035889 @default.
• W2011840549 creator A5052418086 @default.
• W2011840549 creator A5055245083 @default.
• W2011840549 date "2009-09-01" @default.
• W2011840549 modified "2023-09-26" @default.
• W2011840549 title "Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker" @default.
• W2011840549 cites W1514358514 @default.
• W2011840549 cites W1574987057 @default.
• W2011840549 cites W2005124447 @default.
• W2011840549 cites W2011385858 @default.
• W2011840549 cites W2011717673 @default.
• W2011840549 cites W2024556021 @default.
• W2011840549 cites W2040791923 @default.
• W2011840549 cites W2045419277 @default.
• W2011840549 cites W2048101669 @default.
• W2011840549 cites W2058089355 @default.
• W2011840549 cites W2059744613 @default.
• W2011840549 cites W2065808001 @default.
• W2011840549 cites W2066398665 @default.
• W2011840549 cites W2074633451 @default.
• W2011840549 cites W2081408539 @default.
• W2011840549 cites W2084412104 @default.
• W2011840549 cites W2096793632 @default.
• W2011840549 cites W2111042983 @default.
• W2011840549 cites W2126123282 @default.
• W2011840549 cites W2139284717 @default.
• W2011840549 cites W2141659961 @default.
• W2011840549 cites W215871507 @default.
• W2011840549 cites W2158991606 @default.
• W2011840549 cites W2164546956 @default.
• W2011840549 cites W2188008135 @default.
• W2011840549 cites W2189545157 @default.
• W2011840549 cites W2319555894 @default.
• W2011840549 cites W2589395534 @default.
• W2011840549 cites W2589737880 @default.
• W2011840549 cites W2590375193 @default.
• W2011840549 cites W31233541 @default.
• W2011840549 doi "https://doi.org/10.1016/j.scienta.2009.04.001" @default.
• W2011840549 hasPublicationYear "2009" @default.
• W2011840549 type Work @default.
• W2011840549 sameAs 2011840549 @default.
• W2011840549 citedByCount "63" @default.
• W2011840549 countsByYear W20118405492012 @default.
• W2011840549 countsByYear W20118405492013 @default.
• W2011840549 countsByYear W20118405492014 @default.
• W2011840549 countsByYear W20118405492015 @default.
• W2011840549 countsByYear W20118405492017 @default.
• W2011840549 countsByYear W20118405492018 @default.
• W2011840549 countsByYear W20118405492019 @default.
• W2011840549 countsByYear W20118405492020 @default.
• W2011840549 countsByYear W20118405492021 @default.
• W2011840549 countsByYear W20118405492022 @default.
• W2011840549 countsByYear W20118405492023 @default.
• W2011840549 crossrefType "journal-article" @default.
• W2011840549 hasAuthorship W2011840549A5006002742 @default.
• W2011840549 hasAuthorship W2011840549A5008896828 @default.
• W2011840549 hasAuthorship W2011840549A5017792944 @default.
• W2011840549 hasAuthorship W2011840549A5040035889 @default.
• W2011840549 hasAuthorship W2011840549A5052418086 @default.
• W2011840549 hasAuthorship W2011840549A5055245083 @default.
• W2011840549 hasConcept C102230213 @default.
• W2011840549 hasConcept C104317684 @default.
• W2011840549 hasConcept C122172344 @default.
• W2011840549 hasConcept C144027150 @default.
• W2011840549 hasConcept C153911025 @default.
• W2011840549 hasConcept C204241405 @default.
• W2011840549 hasConcept C2775862043 @default.
• W2011840549 hasConcept C2776162087 @default.
• W2011840549 hasConcept C2776460866 @default.
• W2011840549 hasConcept C2778495000 @default.
• W2011840549 hasConcept C2778722857 @default.
• W2011840549 hasConcept C2779656422 @default.
• W2011840549 hasConcept C2910266619 @default.
• W2011840549 hasConcept C523546767 @default.
• W2011840549 hasConcept C54355233 @default.
• W2011840549 hasConcept C83082669 @default.
• W2011840549 hasConcept C86803240 @default.
• W2011840549 hasConcept C89423630 @default.
• W2011840549 hasConceptScore W2011840549C102230213 @default.
• W2011840549 hasConceptScore W2011840549C104317684 @default.
• W2011840549 hasConceptScore W2011840549C122172344 @default.
• W2011840549 hasConceptScore W2011840549C144027150 @default.
• W2011840549 hasConceptScore W2011840549C153911025 @default.
• W2011840549 hasConceptScore W2011840549C204241405 @default.
• W2011840549 hasConceptScore W2011840549C2775862043 @default.
• W2011840549 hasConceptScore W2011840549C2776162087 @default.
• W2011840549 hasConceptScore W2011840549C2776460866 @default.
• W2011840549 hasConceptScore W2011840549C2778495000 @default.
• W2011840549 hasConceptScore W2011840549C2778722857 @default.
• W2011840549 hasConceptScore W2011840549C2779656422 @default.
• W2011840549 hasConceptScore W2011840549C2910266619 @default.
• W2011840549 hasConceptScore W2011840549C523546767 @default.
• W2011840549 hasConceptScore W2011840549C54355233 @default.

• next