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• W2011852020 abstract "When HeLa cells were labeled with either [3H]adenosine or H332PO4, the absolute frequency of m6A (N-6-methyladenosine) decreased 2 to 2.5-fold between a short and long label. The frequency of caps (m7GpppXmpYp) remained constant at a level which suggests that most if not all mRNAs are capped in either a short or a long label. Since the inhibition of heterogeneous nuclear RNA synthesis with either actinomycin D or 5,6-dichloro-I-β-d-ribofuranosyl-benzimidazole halted the labeling of both m6A and caps within 10 to 15 minutes and no direct cytoplasmic addition of m6A could be detected, m6A must be lost faster than caps from the cytoplasmic mRNA. This preferential loss could be due to one or more of the following: (1) a m6A demethylase; (2) excision of m6A containing sequences followed by splicing of the remaining parts of the mRNA; or (3) a minority of the mRNA with a high frequency of m6A leaving the cytoplasmic compartment rapidly, presumably by being degraded." @default.
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• W2011852020 date "1978-09-01" @default.
• W2011852020 modified "2023-10-06" @default.
• W2011852020 title "The absolute frequency of labeled N-6-methyladenosine in HeLa cell messenger RNA decreases with label time" @default.
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• W2011852020 doi "https://doi.org/10.1016/0022-2836(78)90183-3" @default.
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