Matches in SemOpenAlex for { <https://semopenalex.org/work/W2011876819> ?p ?o ?g. }
- W2011876819 endingPage "4446" @default.
- W2011876819 startingPage "4435" @default.
- W2011876819 abstract "Abstract Telomerase activity is elevated in more than 85% of cancer cells and absent in most of the normal cells and thus represents a potential cancer biomarker. We report its measurement in colon and bladder cancer cells captured using antibody‐coated magnetic beads. The cells are lysed and telomerase activity is detected using a biosensor assay that employs an oligonucleotide containing the telomerase recognition sequence also covalently coupled to magnetic beads. Telomerase activity is measured by the incorporation of multiple biotinylated nucleotides at the 3′‐end of the oligonucleotide strands during elongation which are then reacted with streptavidin‐conjugated horseradish peroxidase. A luminescent signal is generated when hydrogen peroxidase is added in the presence of luminol and a signal enhancer. LOD experiments confirm sensitivity down to ten cancer cell equivalents. The telomerase assay reliably identified patient samples considered by an independent pathological review to contain cancer cells. Samples from normal healthy volunteers were all telomerase negative. The assay, which is amenable to automation, demonstrated high sensitivity and specificity in a small clinical cohort, making it of potential benefit as a first line assay for detection and monitoring of colon and bladder cancer." @default.
- W2011876819 created "2016-06-24" @default.
- W2011876819 creator A5003702187 @default.
- W2011876819 creator A5010759705 @default.
- W2011876819 creator A5014061550 @default.
- W2011876819 creator A5031792222 @default.
- W2011876819 creator A5037001639 @default.
- W2011876819 creator A5045089939 @default.
- W2011876819 creator A5057682060 @default.
- W2011876819 creator A5074341523 @default.
- W2011876819 creator A5082697210 @default.
- W2011876819 creator A5082935359 @default.
- W2011876819 date "2007-11-26" @default.
- W2011876819 modified "2023-10-17" @default.
- W2011876819 title "A novel magnetic bead‐based assay with high sensitivity and selectivity for analysis of telomerase in exfoliated cells from patients with bladder and colon cancer" @default.
- W2011876819 cites W1516272650 @default.
- W2011876819 cites W1517663229 @default.
- W2011876819 cites W1533210170 @default.
- W2011876819 cites W1729812787 @default.
- W2011876819 cites W1775829520 @default.
- W2011876819 cites W1908015118 @default.
- W2011876819 cites W1964819590 @default.
- W2011876819 cites W1975880465 @default.
- W2011876819 cites W1990837388 @default.
- W2011876819 cites W1992169010 @default.
- W2011876819 cites W1996207491 @default.
- W2011876819 cites W2000798721 @default.
- W2011876819 cites W2000971601 @default.
- W2011876819 cites W2001899785 @default.
- W2011876819 cites W2004199553 @default.
- W2011876819 cites W2004521761 @default.
- W2011876819 cites W2007419319 @default.
- W2011876819 cites W2010444010 @default.
- W2011876819 cites W2013364403 @default.
- W2011876819 cites W2016219977 @default.
- W2011876819 cites W2017684157 @default.
- W2011876819 cites W2018097391 @default.
- W2011876819 cites W2021742786 @default.
- W2011876819 cites W2021758398 @default.
- W2011876819 cites W2023458939 @default.
- W2011876819 cites W2033413193 @default.
- W2011876819 cites W2036946548 @default.
- W2011876819 cites W2037602734 @default.
- W2011876819 cites W2044296969 @default.
- W2011876819 cites W2045627313 @default.
- W2011876819 cites W2048340114 @default.
- W2011876819 cites W2053076013 @default.
- W2011876819 cites W2055278015 @default.
- W2011876819 cites W2062395030 @default.
- W2011876819 cites W2064482102 @default.
- W2011876819 cites W2071567398 @default.
- W2011876819 cites W2078280108 @default.
- W2011876819 cites W2078413373 @default.
- W2011876819 cites W2081095585 @default.
- W2011876819 cites W2086901270 @default.
- W2011876819 cites W2087376037 @default.
- W2011876819 cites W2091741557 @default.
- W2011876819 cites W2093506065 @default.
- W2011876819 cites W2094229819 @default.
- W2011876819 cites W2094667252 @default.
- W2011876819 cites W2095445389 @default.
- W2011876819 cites W2098958871 @default.
- W2011876819 cites W2102974654 @default.
- W2011876819 cites W2105150199 @default.
- W2011876819 cites W2106448082 @default.
- W2011876819 cites W2109561158 @default.
- W2011876819 cites W2109855241 @default.
- W2011876819 cites W2109970382 @default.
- W2011876819 cites W2110752622 @default.
- W2011876819 cites W2123346671 @default.
- W2011876819 cites W2126415088 @default.
- W2011876819 cites W2135881279 @default.
- W2011876819 cites W2139793739 @default.
- W2011876819 cites W2144118457 @default.
- W2011876819 cites W2148262546 @default.
- W2011876819 cites W2150209800 @default.
- W2011876819 cites W2157487685 @default.
- W2011876819 cites W2163203082 @default.
- W2011876819 cites W2165918134 @default.
- W2011876819 cites W2166692456 @default.
- W2011876819 cites W2172226735 @default.
- W2011876819 cites W2221618400 @default.
- W2011876819 cites W2312358672 @default.
- W2011876819 cites W2332749168 @default.
- W2011876819 cites W2410669078 @default.
- W2011876819 cites W2439390340 @default.
- W2011876819 cites W2803327664 @default.
- W2011876819 cites W3143234154 @default.
- W2011876819 cites W4376489876 @default.
- W2011876819 doi "https://doi.org/10.1002/elps.200600829" @default.
- W2011876819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17987629" @default.
- W2011876819 hasPublicationYear "2007" @default.
- W2011876819 type Work @default.
- W2011876819 sameAs 2011876819 @default.
- W2011876819 citedByCount "10" @default.
- W2011876819 countsByYear W20118768192012 @default.
- W2011876819 countsByYear W20118768192015 @default.
- W2011876819 countsByYear W20118768192016 @default.