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- W2011949494 abstract "Among the many approaches to the separation of cells, sedimentation either at unit gravity or with a centrifugal force has been widely used. Since the quality of the suspensions of cells prior to sedimentation greatly influences the separation, it is important to evaluate several methods for the dispersion of solid tissues to obtain suspensions of viable, single cells. The optimal approach to a particular tissue varies as a function of organ, donor species and age, and other experimental variables. There are two kinds of sedimentation that can be used for cell separation in continuous gradients: velocity sedimentation and isopycnic sedimentation. Isopycnic sedimentation accomplishes cell separations according to the different densities of cells. There are four commonly used forms of velocity sedimentation: sedimentation at unit gravity, isokinetic sedimentation, sedimentation in a reorienting zonal rotor, and elutriation. The choice of method for the separation of particular kinds of cells will depend on the needs of the investigator, including the number of cells desired, the cost, and the need for work under sterile conditions." @default.
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- W2011949494 date "1991-06-01" @default.
- W2011949494 modified "2023-09-26" @default.
- W2011949494 title "Sedimentation for the separation of cells" @default.
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- W2011949494 doi "https://doi.org/10.1016/s1046-2023(05)80060-x" @default.
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