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- W2011980902 abstract "Abstract The procedure for separation of polyoma DNA and mouse embryo cell DNA by chromatography on MAK columns (Dulbecco et al. , 1965) has been extended. Native mouse embryo DNA was found to elute from MAK columns at concentrations of NaCl between 0.55 and 0.65 M , whereas heat-denatured mouse embryo DNA was not eluted even with 0.8 M NaCl. Heat-treated polyoma DNA was eluted from MAK columns at the same concentration of NaCl (0.53–0.57 M ) which eluted native virus DNA. Using these properties of cell and viral DNA, the nature of the DNA synthesized in mouse embryo cells replicating polyoma virus has been examined. In exponentially growing cells, in which nearly all infected cells make virus, the DNA synthesized up to 12 hours after infection (i.e., during eclipse) was all cell DNA. After 12 hours the synthesis of cell DNA decreased to reach zero at about 18–20 hours after infection. Viral DNA synthesis began at about 12 hours after infection and increased progressively so that by 18–20 hours after infection all the DNA synthesized in the infected cultures was viral DNA." @default.
- W2011980902 created "2016-06-24" @default.
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- W2011980902 date "1966-04-01" @default.
- W2011980902 modified "2023-09-27" @default.
- W2011980902 title "Deoxyribonucleic acid synthesis in cells replicating polyoma virus" @default.
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- W2011980902 doi "https://doi.org/10.1016/0042-6822(66)90247-9" @default.
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