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- W2012000266 abstract "This study assessed the morphogenic response of in vitro cultured zygotic embryos obtained from Pseu¬dotsuga menziesii var. glauca seed collected in Tlaxcala, Mexico and stored for one year. Seeds were disinfected with detergent and H2O2 (3 % v/v) for 48 h under stirring at 50 rpm and cultured on Murashige and Skoog (1962) medium without regulators. Germination occurred after seven days and then the explants were subcultured on MS medium with 2,4-D (3 mg.L-1) and BA (1 mg.L-1). With the calluses obtained on HS medium, three ABA concentrations intended to promote formation of embryonic structures were evaluated. The best treatment was with a concentration of 10.0 mg.L-1 (P" @default.
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- W2012000266 date "2011-08-01" @default.
- W2012000266 modified "2023-10-13" @default.
- W2012000266 title "MORFOGÉNESIS IN VITRO DE Pseudotsuga menziesii VAR. glauca" @default.
- W2012000266 doi "https://doi.org/10.5154/r.rchscfa.2010.04.024" @default.
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