FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012006504> ?p ?o ?g. }

• W2012006504 endingPage "150" @default.
• W2012006504 startingPage "143" @default.
• W2012006504 abstract "Integrating lentiviral vectors based on the human immunodeficiency virus type-1 (HIV-1) can transduce quiescent cells, which in lung account for almost 95% of the epithelial cell population. Pseudotyping lentiviral vectors with the envelope glycoprotein from the Ebola Zaire virus, the lymphocytic choriomeningitis virus (LCMV), the Mokola virus, and the vesicular stomatitis virus (VSV-G) resulted in transduction of mouse alveolar epithelium, but gene expression in the lung of C57BL/6 and BALB/c mice waned within 90 days of vector injection. Intratracheal delivery of the four pseudotyped lentiviral vectors resulted in transgene-specific T-cell activation in both mouse strains, albeit lower than that achieved by intramuscular injection of the vectors. We performed an adoptive transfer of luciferase-specific T cells, isolated from spleen or lung of donor mice injected with VSV-G-pseudotyped lentivirus vector expressing luciferase into the muscle or lung, respectively, into recipient recombination-activating gene (RAG)–deficient mice transduced in lung with adenovirus expressing firefly luciferase (ffluc2). Gene expression declined within 7 days of adoptive transfer approaching background levels by day 36. Taken together, our results suggest that the loss of transduced cells in lung is due to VSV-G.HIV vector–mediated activation of transgene-specific T cells rather than as result of normal turnover of airway cells. Integrating lentiviral vectors based on the human immunodeficiency virus type-1 (HIV-1) can transduce quiescent cells, which in lung account for almost 95% of the epithelial cell population. Pseudotyping lentiviral vectors with the envelope glycoprotein from the Ebola Zaire virus, the lymphocytic choriomeningitis virus (LCMV), the Mokola virus, and the vesicular stomatitis virus (VSV-G) resulted in transduction of mouse alveolar epithelium, but gene expression in the lung of C57BL/6 and BALB/c mice waned within 90 days of vector injection. Intratracheal delivery of the four pseudotyped lentiviral vectors resulted in transgene-specific T-cell activation in both mouse strains, albeit lower than that achieved by intramuscular injection of the vectors. We performed an adoptive transfer of luciferase-specific T cells, isolated from spleen or lung of donor mice injected with VSV-G-pseudotyped lentivirus vector expressing luciferase into the muscle or lung, respectively, into recipient recombination-activating gene (RAG)–deficient mice transduced in lung with adenovirus expressing firefly luciferase (ffluc2). Gene expression declined within 7 days of adoptive transfer approaching background levels by day 36. Taken together, our results suggest that the loss of transduced cells in lung is due to VSV-G.HIV vector–mediated activation of transgene-specific T cells rather than as result of normal turnover of airway cells." @default.
• W2012006504 created "2016-06-24" @default.
• W2012006504 creator A5044822697 @default.
• W2012006504 creator A5051344823 @default.
• W2012006504 creator A5054554818 @default.
• W2012006504 creator A5060948677 @default.
• W2012006504 date "2010-01-01" @default.
• W2012006504 modified "2023-09-26" @default.
• W2012006504 title "Activation of Transgene-specific T Cells Following Lentivirus-mediated Gene Delivery to Mouse Lung" @default.
• W2012006504 cites W1485992471 @default.
• W2012006504 cites W1548280824 @default.
• W2012006504 cites W1566969252 @default.
• W2012006504 cites W1606395976 @default.
• W2012006504 cites W1963799836 @default.
• W2012006504 cites W1964018172 @default.
• W2012006504 cites W1991519731 @default.
• W2012006504 cites W1999053786 @default.
• W2012006504 cites W2003999802 @default.
• W2012006504 cites W2009761723 @default.
• W2012006504 cites W2012104351 @default.
• W2012006504 cites W2016149094 @default.
• W2012006504 cites W2017994016 @default.
• W2012006504 cites W2020748860 @default.
• W2012006504 cites W2022501300 @default.
• W2012006504 cites W2034210638 @default.
• W2012006504 cites W2038122139 @default.
• W2012006504 cites W2046870756 @default.
• W2012006504 cites W2058973580 @default.
• W2012006504 cites W2072771134 @default.
• W2012006504 cites W2076157508 @default.
• W2012006504 cites W2077658669 @default.
• W2012006504 cites W2079555292 @default.
• W2012006504 cites W2082139926 @default.
• W2012006504 cites W2088542333 @default.
• W2012006504 cites W2092170974 @default.
• W2012006504 cites W2121291544 @default.
• W2012006504 cites W2122432632 @default.
• W2012006504 cites W2122868566 @default.
• W2012006504 cites W2124318077 @default.
• W2012006504 cites W2131095976 @default.
• W2012006504 cites W2140489687 @default.
• W2012006504 cites W2145604558 @default.
• W2012006504 cites W2148019580 @default.
• W2012006504 cites W2151902657 @default.
• W2012006504 cites W2156119419 @default.
• W2012006504 cites W2164195236 @default.
• W2012006504 cites W2164316881 @default.
• W2012006504 doi "https://doi.org/10.1038/mt.2009.190" @default.
• W2012006504 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2839217" @default.
• W2012006504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19724265" @default.
• W2012006504 hasPublicationYear "2010" @default.
• W2012006504 type Work @default.
• W2012006504 sameAs 2012006504 @default.
• W2012006504 citedByCount "33" @default.
• W2012006504 countsByYear W20120065042012 @default.
• W2012006504 countsByYear W20120065042013 @default.
• W2012006504 countsByYear W20120065042014 @default.
• W2012006504 countsByYear W20120065042015 @default.
• W2012006504 countsByYear W20120065042017 @default.
• W2012006504 countsByYear W20120065042018 @default.
• W2012006504 countsByYear W20120065042021 @default.
• W2012006504 countsByYear W20120065042022 @default.
• W2012006504 countsByYear W20120065042023 @default.
• W2012006504 crossrefType "journal-article" @default.
• W2012006504 hasAuthorship W2012006504A5044822697 @default.
• W2012006504 hasAuthorship W2012006504A5051344823 @default.
• W2012006504 hasAuthorship W2012006504A5054554818 @default.
• W2012006504 hasAuthorship W2012006504A5060948677 @default.
• W2012006504 hasBestOaLocation W20120065041 @default.
• W2012006504 hasConcept C102230213 @default.
• W2012006504 hasConcept C104317684 @default.
• W2012006504 hasConcept C111599444 @default.
• W2012006504 hasConcept C135983454 @default.
• W2012006504 hasConcept C15152581 @default.
• W2012006504 hasConcept C153911025 @default.
• W2012006504 hasConcept C159047783 @default.
• W2012006504 hasConcept C2522874641 @default.
• W2012006504 hasConcept C2779057330 @default.
• W2012006504 hasConcept C2779690655 @default.
• W2012006504 hasConcept C2780727368 @default.
• W2012006504 hasConcept C2908647359 @default.
• W2012006504 hasConcept C32470452 @default.
• W2012006504 hasConcept C40767141 @default.
• W2012006504 hasConcept C55493867 @default.
• W2012006504 hasConcept C71924100 @default.
• W2012006504 hasConcept C86803240 @default.
• W2012006504 hasConcept C99454951 @default.
• W2012006504 hasConceptScore W2012006504C102230213 @default.
• W2012006504 hasConceptScore W2012006504C104317684 @default.
• W2012006504 hasConceptScore W2012006504C111599444 @default.
• W2012006504 hasConceptScore W2012006504C135983454 @default.
• W2012006504 hasConceptScore W2012006504C15152581 @default.
• W2012006504 hasConceptScore W2012006504C153911025 @default.
• W2012006504 hasConceptScore W2012006504C159047783 @default.
• W2012006504 hasConceptScore W2012006504C2522874641 @default.
• W2012006504 hasConceptScore W2012006504C2779057330 @default.
• W2012006504 hasConceptScore W2012006504C2779690655 @default.
• W2012006504 hasConceptScore W2012006504C2780727368 @default.

• next