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- W2012010628 abstract "Bovine and canine chromogranin A were extracted and purified from each specie's adrenal glands. Isolated bovine 70 kDa protein showed 100% identity to bovine CgA reported previously, whereas isolated canine 68 kDa protein showed 83.3% identity to bovine CgA by the NH2-terminal amino acid sequence analysis. Rabbit antibody to purified bovine protein (CgA) was found to immunologically cross-reatced with purified canine protein (CgA). In sandwich ELISA with anti-bovine CgA, concentration-dependent curves were obtained ranging from 0.3 to 20 μg/ml for canine CgA. From these findings, sandwich ELISA with anti-bovine CgA is found to be useful to determine the concentration of canine CgA." @default.
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- W2012010628 date "2005-01-01" @default.
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- W2012010628 title "Emzyme-Linked Immunosorbent Assay for Detection of Canine Chromogranin A by Use of Immunological Cross-Reactivity of Rabbit Anti-Bovine Chromogranin A Antibody" @default.
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- W2012010628 doi "https://doi.org/10.1292/jvms.67.535" @default.
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