Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012023443> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2012023443 abstract "Hydroxy fatty acids (HFAs) are high-added-value compounds, which are incorporated in polymers, lubricants, emulsifiers and stabilizers and have potential medicinal use. In nature, HFAs are regio-specifically synthesized by several enzymes, including P450 monooxygenases, lipoxygenases, hydratases, 12-hydroxylases, and diol synthases. The growing demand for HFAs warrants the development of simple and efficient analytical methods that enable high-throughput detection of the hydroxylated product in the presence of its unsaturated precursor. Herein a novel high-throughput assay for the detection of alcohols is described using oleate hydratase (OHase, EC 4.2.1.53) from Elizabethkingia meningoseptica as the model enzyme. The developed assay is based on the selective spectrophotometric detection of alkyl nitrites formed upon the reaction between the hydroxyl group and nitrous acid. The assay proved to discriminate between unsaturated fatty acids as well as small cyclic and acyclic unsaturated alkenes and their corresponding alcohols. Lower detection limits were 1.5-3 mM with excellent Z'-factors. Enzymatic reactions using OHase with oleic acid resulted in somewhat lower Z-factors for various enzyme preparations. This small scale assay can enable fast discovery of new microorganisms or improved enzymes from mutant libraries and will be useful for biocatalytic strategies involving fatty acid (de)hydrating enzymes." @default.
- W2012023443 created "2016-06-24" @default.
- W2012023443 creator A5026903144 @default.
- W2012023443 creator A5028443868 @default.
- W2012023443 creator A5029539749 @default.
- W2012023443 creator A5061250054 @default.
- W2012023443 date "2014-02-12" @default.
- W2012023443 modified "2023-09-23" @default.
- W2012023443 title "Enzymatic hydration activity assessed by selective spectrophotometric detection of alcohols: A novel screening assay using oleate hydratase as a model enzyme" @default.
- W2012023443 cites W1969951206 @default.
- W2012023443 cites W1970030716 @default.
- W2012023443 cites W1975108319 @default.
- W2012023443 cites W1991001144 @default.
- W2012023443 cites W2011178157 @default.
- W2012023443 cites W2012202465 @default.
- W2012023443 cites W2038359733 @default.
- W2012023443 cites W2039028644 @default.
- W2012023443 cites W2041721973 @default.
- W2012023443 cites W2044349991 @default.
- W2012023443 cites W2061870697 @default.
- W2012023443 cites W2068838190 @default.
- W2012023443 cites W2082613114 @default.
- W2012023443 cites W2092613007 @default.
- W2012023443 cites W2094029958 @default.
- W2012023443 cites W2114456393 @default.
- W2012023443 cites W2114556103 @default.
- W2012023443 cites W2126506795 @default.
- W2012023443 cites W2321205391 @default.
- W2012023443 cites W4243236983 @default.
- W2012023443 doi "https://doi.org/10.1002/biot.201300412" @default.
- W2012023443 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24449561" @default.
- W2012023443 hasPublicationYear "2014" @default.
- W2012023443 type Work @default.
- W2012023443 sameAs 2012023443 @default.
- W2012023443 citedByCount "6" @default.
- W2012023443 countsByYear W20120234432014 @default.
- W2012023443 countsByYear W20120234432015 @default.
- W2012023443 countsByYear W20120234432018 @default.
- W2012023443 countsByYear W20120234432021 @default.
- W2012023443 crossrefType "journal-article" @default.
- W2012023443 hasAuthorship W2012023443A5026903144 @default.
- W2012023443 hasAuthorship W2012023443A5028443868 @default.
- W2012023443 hasAuthorship W2012023443A5029539749 @default.
- W2012023443 hasAuthorship W2012023443A5061250054 @default.
- W2012023443 hasConcept C13002179 @default.
- W2012023443 hasConcept C178790620 @default.
- W2012023443 hasConcept C181199279 @default.
- W2012023443 hasConcept C185592680 @default.
- W2012023443 hasConcept C2781403372 @default.
- W2012023443 hasConcept C43617362 @default.
- W2012023443 hasConcept C51323132 @default.
- W2012023443 hasConcept C526171541 @default.
- W2012023443 hasConcept C543025807 @default.
- W2012023443 hasConcept C55493867 @default.
- W2012023443 hasConceptScore W2012023443C13002179 @default.
- W2012023443 hasConceptScore W2012023443C178790620 @default.
- W2012023443 hasConceptScore W2012023443C181199279 @default.
- W2012023443 hasConceptScore W2012023443C185592680 @default.
- W2012023443 hasConceptScore W2012023443C2781403372 @default.
- W2012023443 hasConceptScore W2012023443C43617362 @default.
- W2012023443 hasConceptScore W2012023443C51323132 @default.
- W2012023443 hasConceptScore W2012023443C526171541 @default.
- W2012023443 hasConceptScore W2012023443C543025807 @default.
- W2012023443 hasConceptScore W2012023443C55493867 @default.
- W2012023443 hasLocation W20120234431 @default.
- W2012023443 hasLocation W20120234432 @default.
- W2012023443 hasOpenAccess W2012023443 @default.
- W2012023443 hasPrimaryLocation W20120234431 @default.
- W2012023443 hasRelatedWork W1586135266 @default.
- W2012023443 hasRelatedWork W1589208118 @default.
- W2012023443 hasRelatedWork W1978392142 @default.
- W2012023443 hasRelatedWork W2012023443 @default.
- W2012023443 hasRelatedWork W2074363506 @default.
- W2012023443 hasRelatedWork W2138146016 @default.
- W2012023443 hasRelatedWork W2138772345 @default.
- W2012023443 hasRelatedWork W2159186935 @default.
- W2012023443 hasRelatedWork W2317260126 @default.
- W2012023443 hasRelatedWork W1993836633 @default.
- W2012023443 isParatext "false" @default.
- W2012023443 isRetracted "false" @default.
- W2012023443 magId "2012023443" @default.
- W2012023443 workType "article" @default.