Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012030237> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2012030237 endingPage "141" @default.
- W2012030237 startingPage "127" @default.
- W2012030237 abstract "Digital imaging fluorescence microscopy (DIFM) of tissue sections was used to quantify uptake of labelled plasma proteins by the arterial wall. Several aspects of the measuring system were investigated so that absolute tracer concentrations and their local variation could be derived from digitized images. These investigations may be relevant to other studies employing DIFM. Nonlinearities were found to arise from offsets in the video digitizers, from background fluorescence and stray light within the microscope and from the transfer characteristics of the intensified CCD camera. Camera gain controls showed complex behaviour. Camera output fell substantially for several hours after switching on and was affected by room temperature. Large spatial variations in response were caused by the geometry of the microscope optics and by the image intensifier. However, the ratios between areas were not affected by light intensity or camera gain settings. Measured intensities were independent of the depthwise location of fluorophores within tissue sections but they were affected by the emission from objects outside the measuring area. Photobleaching of tracer varied significantly over the range of excitation intensities and durations used but was not concentration dependent. Methods used to correct these effects and obtain a uniform, linear and constant relationship between concentration and grey level are described. Using the system and appropriate corrections, in vivo uptake of sulphorhodamine-B-labelled serum albumin by the rabbit aortic wall was investigated. Results obtained for the mean uptake of tracer and its local variation were quantitatively similar to those previously obtained with nonmicroscopic methods." @default.
- W2012030237 created "2016-06-24" @default.
- W2012030237 creator A5034250929 @default.
- W2012030237 creator A5076933022 @default.
- W2012030237 creator A5084709068 @default.
- W2012030237 date "1994-02-01" @default.
- W2012030237 modified "2023-09-28" @default.
- W2012030237 title "Measurement of absolute tracer concentrations in tissue sections by using digital imaging fluorescence microscopy. Application to the study of plasma protein uptake by the arterial wall" @default.
- W2012030237 cites W1575381933 @default.
- W2012030237 cites W1964355587 @default.
- W2012030237 cites W1970914240 @default.
- W2012030237 cites W1974554617 @default.
- W2012030237 cites W1985665254 @default.
- W2012030237 cites W1994512250 @default.
- W2012030237 cites W2009275656 @default.
- W2012030237 cites W2027082111 @default.
- W2012030237 cites W2037321679 @default.
- W2012030237 cites W2040988238 @default.
- W2012030237 cites W2054097605 @default.
- W2012030237 cites W2055058797 @default.
- W2012030237 cites W2141798937 @default.
- W2012030237 cites W2169124471 @default.
- W2012030237 cites W4233881130 @default.
- W2012030237 cites W930163778 @default.
- W2012030237 doi "https://doi.org/10.1111/j.1365-2818.1994.tb03435.x" @default.
- W2012030237 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8169950" @default.
- W2012030237 hasPublicationYear "1994" @default.
- W2012030237 type Work @default.
- W2012030237 sameAs 2012030237 @default.
- W2012030237 citedByCount "17" @default.
- W2012030237 countsByYear W20120302372012 @default.
- W2012030237 crossrefType "journal-article" @default.
- W2012030237 hasAuthorship W2012030237A5034250929 @default.
- W2012030237 hasAuthorship W2012030237A5076933022 @default.
- W2012030237 hasAuthorship W2012030237A5084709068 @default.
- W2012030237 hasConcept C113196181 @default.
- W2012030237 hasConcept C120665830 @default.
- W2012030237 hasConcept C121332964 @default.
- W2012030237 hasConcept C136229726 @default.
- W2012030237 hasConcept C147080431 @default.
- W2012030237 hasConcept C169274487 @default.
- W2012030237 hasConcept C185544564 @default.
- W2012030237 hasConcept C185592680 @default.
- W2012030237 hasConcept C192562407 @default.
- W2012030237 hasConcept C2778863792 @default.
- W2012030237 hasConcept C31332276 @default.
- W2012030237 hasConcept C43617362 @default.
- W2012030237 hasConcept C67649825 @default.
- W2012030237 hasConcept C69357855 @default.
- W2012030237 hasConcept C71924100 @default.
- W2012030237 hasConcept C91881484 @default.
- W2012030237 hasConcept C93038891 @default.
- W2012030237 hasConcept C97355855 @default.
- W2012030237 hasConceptScore W2012030237C113196181 @default.
- W2012030237 hasConceptScore W2012030237C120665830 @default.
- W2012030237 hasConceptScore W2012030237C121332964 @default.
- W2012030237 hasConceptScore W2012030237C136229726 @default.
- W2012030237 hasConceptScore W2012030237C147080431 @default.
- W2012030237 hasConceptScore W2012030237C169274487 @default.
- W2012030237 hasConceptScore W2012030237C185544564 @default.
- W2012030237 hasConceptScore W2012030237C185592680 @default.
- W2012030237 hasConceptScore W2012030237C192562407 @default.
- W2012030237 hasConceptScore W2012030237C2778863792 @default.
- W2012030237 hasConceptScore W2012030237C31332276 @default.
- W2012030237 hasConceptScore W2012030237C43617362 @default.
- W2012030237 hasConceptScore W2012030237C67649825 @default.
- W2012030237 hasConceptScore W2012030237C69357855 @default.
- W2012030237 hasConceptScore W2012030237C71924100 @default.
- W2012030237 hasConceptScore W2012030237C91881484 @default.
- W2012030237 hasConceptScore W2012030237C93038891 @default.
- W2012030237 hasConceptScore W2012030237C97355855 @default.
- W2012030237 hasIssue "2" @default.
- W2012030237 hasLocation W20120302371 @default.
- W2012030237 hasLocation W20120302372 @default.
- W2012030237 hasOpenAccess W2012030237 @default.
- W2012030237 hasPrimaryLocation W20120302371 @default.
- W2012030237 hasRelatedWork W1598789855 @default.
- W2012030237 hasRelatedWork W1980698430 @default.
- W2012030237 hasRelatedWork W2042460426 @default.
- W2012030237 hasRelatedWork W2046288440 @default.
- W2012030237 hasRelatedWork W2101336703 @default.
- W2012030237 hasRelatedWork W2819800 @default.
- W2012030237 hasRelatedWork W3133220949 @default.
- W2012030237 hasRelatedWork W3144685119 @default.
- W2012030237 hasRelatedWork W4241546789 @default.
- W2012030237 hasRelatedWork W1714477937 @default.
- W2012030237 hasVolume "173" @default.
- W2012030237 isParatext "false" @default.
- W2012030237 isRetracted "false" @default.
- W2012030237 magId "2012030237" @default.
- W2012030237 workType "article" @default.