FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012030256> ?p ?o ?g. }

• W2012030256 endingPage "978" @default.
• W2012030256 startingPage "967" @default.
• W2012030256 abstract "Active immunotherapy for cancer is an accepted treatment modality aiming to reinforce the T-cell response to cancer. T-cell reactivity is measured by various assays and used to guide the clinical development of immunotherapeutics. However, data obtained across different institutions may vary substantially making comparative conclusions difficult. The Cancer Immunotherapy Immunoguiding Program organizes proficiency panels to identify key parameters influencing the outcome of commonly used T-cell assays followed by harmonization. Our successes with IFNγ-ELISPOT and peptide HLA multimer analysis have led to the current study on intracellular cytokine staining (ICS). We report the results of three successive panels evaluating this assay. At the beginning, 3 out of 9 participants (33 %) were able to detect >6 out of 8 known virus-specific T-cell responses in peripheral blood of healthy individuals. This increased to 50 % of the laboratories in the second phase. The reported percentages of cytokine-producing T cells by the different laboratories were highly variable with coefficients of variation well over 60 %. Variability could partially be explained by protocol-related differences in background cytokine production leading to sub-optimal signal-to-noise ratios. The large number of protocol variables prohibited identification of prime guidelines to harmonize the assays. In addition, the gating strategy used to identify reactive T cells had a major impact on assay outcome. Subsequent harmonization of the gating strategy considerably reduced the variability within the group of participants. In conclusion, we propose that first basic guidelines should be applied for gating in ICS experiments before harmonizing assay protocol variables." @default.
• W2012030256 created "2016-06-24" @default.
• W2012030256 creator A5003613132 @default.
• W2012030256 creator A5018370728 @default.
• W2012030256 creator A5026744127 @default.
• W2012030256 creator A5036257821 @default.
• W2012030256 creator A5041281028 @default.
• W2012030256 creator A5069267169 @default.
• W2012030256 creator A5072238075 @default.
• W2012030256 date "2012-05-22" @default.
• W2012030256 modified "2023-10-17" @default.
• W2012030256 title "Harmonization of the intracellular cytokine staining assay" @default.
• W2012030256 cites W146169322 @default.
• W2012030256 cites W1497374306 @default.
• W2012030256 cites W1552549775 @default.
• W2012030256 cites W1819834770 @default.
• W2012030256 cites W1941148137 @default.
• W2012030256 cites W1989486137 @default.
• W2012030256 cites W1997315475 @default.
• W2012030256 cites W2002662941 @default.
• W2012030256 cites W2014985358 @default.
• W2012030256 cites W2022990363 @default.
• W2012030256 cites W2023244389 @default.
• W2012030256 cites W2025558159 @default.
• W2012030256 cites W2046682570 @default.
• W2012030256 cites W2058078577 @default.
• W2012030256 cites W2066972199 @default.
• W2012030256 cites W2078338369 @default.
• W2012030256 cites W2090001110 @default.
• W2012030256 cites W2093264896 @default.
• W2012030256 cites W2097995306 @default.
• W2012030256 cites W2103959341 @default.
• W2012030256 cites W2117692326 @default.
• W2012030256 cites W2142300779 @default.
• W2012030256 cites W2149754518 @default.
• W2012030256 cites W2159443496 @default.
• W2012030256 cites W2159926306 @default.
• W2012030256 cites W2166248004 @default.
• W2012030256 cites W2170167391 @default.
• W2012030256 doi "https://doi.org/10.1007/s00262-012-1282-9" @default.
• W2012030256 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3378841" @default.
• W2012030256 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22714399" @default.
• W2012030256 hasPublicationYear "2012" @default.
• W2012030256 type Work @default.
• W2012030256 sameAs 2012030256 @default.
• W2012030256 citedByCount "43" @default.
• W2012030256 countsByYear W20120302562012 @default.
• W2012030256 countsByYear W20120302562013 @default.
• W2012030256 countsByYear W20120302562014 @default.
• W2012030256 countsByYear W20120302562015 @default.
• W2012030256 countsByYear W20120302562016 @default.
• W2012030256 countsByYear W20120302562017 @default.
• W2012030256 countsByYear W20120302562018 @default.
• W2012030256 countsByYear W20120302562019 @default.
• W2012030256 countsByYear W20120302562020 @default.
• W2012030256 countsByYear W20120302562021 @default.
• W2012030256 countsByYear W20120302562023 @default.
• W2012030256 crossrefType "journal-article" @default.
• W2012030256 hasAuthorship W2012030256A5003613132 @default.
• W2012030256 hasAuthorship W2012030256A5018370728 @default.
• W2012030256 hasAuthorship W2012030256A5026744127 @default.
• W2012030256 hasAuthorship W2012030256A5036257821 @default.
• W2012030256 hasAuthorship W2012030256A5041281028 @default.
• W2012030256 hasAuthorship W2012030256A5069267169 @default.
• W2012030256 hasAuthorship W2012030256A5072238075 @default.
• W2012030256 hasBestOaLocation W20120302561 @default.
• W2012030256 hasConcept C147483822 @default.
• W2012030256 hasConcept C167672396 @default.
• W2012030256 hasConcept C194544171 @default.
• W2012030256 hasConcept C203014093 @default.
• W2012030256 hasConcept C2777701055 @default.
• W2012030256 hasConcept C2778690821 @default.
• W2012030256 hasConcept C2779053233 @default.
• W2012030256 hasConcept C42407357 @default.
• W2012030256 hasConcept C70721500 @default.
• W2012030256 hasConcept C71924100 @default.
• W2012030256 hasConcept C86803240 @default.
• W2012030256 hasConcept C8891405 @default.
• W2012030256 hasConceptScore W2012030256C147483822 @default.
• W2012030256 hasConceptScore W2012030256C167672396 @default.
• W2012030256 hasConceptScore W2012030256C194544171 @default.
• W2012030256 hasConceptScore W2012030256C203014093 @default.
• W2012030256 hasConceptScore W2012030256C2777701055 @default.
• W2012030256 hasConceptScore W2012030256C2778690821 @default.
• W2012030256 hasConceptScore W2012030256C2779053233 @default.
• W2012030256 hasConceptScore W2012030256C42407357 @default.
• W2012030256 hasConceptScore W2012030256C70721500 @default.
• W2012030256 hasConceptScore W2012030256C71924100 @default.
• W2012030256 hasConceptScore W2012030256C86803240 @default.
• W2012030256 hasConceptScore W2012030256C8891405 @default.
• W2012030256 hasIssue "7" @default.
• W2012030256 hasLocation W20120302561 @default.
• W2012030256 hasLocation W20120302562 @default.
• W2012030256 hasLocation W20120302563 @default.
• W2012030256 hasLocation W20120302564 @default.
• W2012030256 hasOpenAccess W2012030256 @default.
• W2012030256 hasPrimaryLocation W20120302561 @default.
• W2012030256 hasRelatedWork W1506959008 @default.

• next