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• W2012038165 abstract "A cell-free system developed from human KB cells was used to transcribe 5.5S RNA from deproteinized adenovirus DNA in vitro. The cell-free RNA synthesis is dependent upon exogenous templates, ribonucleoside triphosphates, and cell-free postmitochondrial supernatant of human KB cells. The synthesis of 5.5S RNA is inhibited only by high levels of alpha-amanitin; therefore it is carried out by RNA polymerase III. The rate of synthesis was linear for at least 2 hr, indicating reinitiation. The 5.5S RNA synthesized in vitro is similar to the corresponding in vivo RNA in size, sequence, and coding region on adenovirus type 2 DNA. In this report is demonstrated in vitro synthesis of a facsimile of an in vivo transcript directed by deproteinized DNA in a mammalian cell-free postmitochondrial supernatant system." @default.
• W2012038165 created "2016-06-24" @default.
• W2012038165 creator A5050027982 @default.
• W2012038165 date "1978-05-01" @default.
• W2012038165 modified "2023-09-26" @default.
• W2012038165 title "Adenovirus DNA-directed transcription of 5.5S RNA in vitro." @default.
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• W2012038165 doi "https://doi.org/10.1073/pnas.75.5.2175" @default.
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