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- W2012040359 abstract "Polysomes synthesizing Type I and II procollagens were isolated from rat calvaria and from a murine chondrosarcoma respectively. All collagenase sensitive polypeptides synthesized in vitro, as well as the collagen specific immunoprecipitated materials were smaller than β components. Polysomes contained nascent peptides consistently smaller than α chains. In low K+ (10mM) nonspecific adsorption of collagen to polysomes and aggregation of polysome were observed. Such effects were reversed in the presence of 250mM K+. We find no evidence supporting the polycistronic mRNA hypothesis for collagen and suggest rather that large polysomal aggregates are artifacts generated at low K+. We propose that collagen is synthesized on polysomes as a precursor polypeptide smaller than 185,000." @default.
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- W2012040359 date "1977-07-01" @default.
- W2012040359 modified "2023-09-27" @default.
- W2012040359 title "Evidence that the messenger RNA for collagen is monocistronic" @default.
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- W2012040359 doi "https://doi.org/10.1016/s0006-291x(77)80158-7" @default.
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