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- W2012045500 abstract "Background We hypothesized that by studying a suspension of a single cell type in various resuscitation fluids, we could compare their effects on parameters associated with cell death. Methods Jurkat cells were suspended in resuscitation fluids. Using flow cytometry, light scatter, phosphatidylserine translocation, propidium iodide uptake, and intracellular H2O2 production were measured. Results Buffer (pH, 7.4) and albumin added to Ringer’s lactate inhibited the adverse changes, including intracellular oxygen free radical production. Oxygen free radical production was variable within the cell population and inhibited by albumin but not other colloids or crystalloids. This correlated well with the ability of albumin to enhance metabolic activity. A flavoprotein inhibitor blocked H2O2 production, suggesting that mitochondria are the source of the H2O2 and the variability. Conclusion Oxygen free radical inhibition by albumin could explain both its beneficial and its harmful effects." @default.
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- W2012045500 date "2004-03-01" @default.
- W2012045500 modified "2023-10-03" @default.
- W2012045500 title "Heterogeneity in the Effect of Albumin and Other Resuscitation Fluids on Intracellular Oxygen Free Radical Production" @default.
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- W2012045500 doi "https://doi.org/10.1097/01.ta.0000112933.83144.23" @default.
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