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- W2012057592 abstract "We report the generation of murine triomas by fusing splenocytes from mice previously immunized with HBsAg ay-subtype and a hybridoma, secreting anti-HBsAg ad-subtype monoclonal antibody, which was rendered HGPRT- by induced mutagenesis with N-methyl-N'nitro-N-nitrosoguanidine. The fusion yielded a 83.8% of hybrids showing the antigen specificity of the parental hybridoma and a 16.1% of bi-specific monoclonal antibodies. One of them, coded as 1C8A5, showing a heavy chain isotype (IgG1/IgG2b) was used as capture reagent in an ultramicro-ELISA. As little as 0.78 I.U. of both HBsAg ad- and ay-subtypes could be realiably detected." @default.
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- W2012057592 date "1992-12-01" @default.
- W2012057592 modified "2023-10-06" @default.
- W2012057592 title "Generation of Murine Triomas Secreting Bi-specific Monoclonal Antibodies That Recognize HBsAGadandaySubtypes" @default.
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- W2012057592 doi "https://doi.org/10.1089/hyb.1992.11.815" @default.
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