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• W2012078049 abstract "The functional properties of RNA polymerase II are modulated by hyperphosphorylation of its unique C-terminal repeat domain (CTD). A number of enzymes with CTD kinase activity have been identified, and correlations between CTD phosphorylation and RNA polymerase II function have been made. Here we describe methods for assaying CTD kinases and for characterizing them enzymologically. In addition we present approaches for studying phosphorylation-mediated behavior of chromosome-associated RNA polymerase II by using CTD-directed, phosphorylation state-sensitive antibodies and in situ localization techniques. The methods described here should, in conjunction with genetic approaches, contribute to elucidating the physiological roles of CTD kinases." @default.
• W2012078049 created "2016-06-24" @default.
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• W2012078049 date "1997-07-01" @default.
• W2012078049 modified "2023-10-17" @default.
• W2012078049 title "Assaying CTD Kinasesin Vitroand Phosphorylation-Modulated Properties of RNA Polymerase IIin Vivo" @default.
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• W2012078049 doi "https://doi.org/10.1006/meth.1997.0478" @default.
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