Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012081039> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2012081039 endingPage "275" @default.
- W2012081039 startingPage "275" @default.
- W2012081039 abstract "Modulation of the nuclear entry of transcription factors (TFs) and chromatin components is a means by which eukaryotic cells can regulate gene expression in response to extracellular signals and the cell cycle during differentiation and development. TFs and chromatin components access the nucleus through nuclear localisation sequences (NLSs), which mediate interaction with components of the cellular nuclear import machinery, such as members of the importin superfamily. The Ca2+-binding protein calmodulin (CaM ) has previously been shown to bind at or near NLSs in several nuclear-localising proteins that have important roles in testis development including the Y chromosome-encoded HMG-domain-carrying chromatin remodelling factor SRY, and related factor SOX9, both of which are key regulators of gonadal development. SRY function in the nucleus of somatic cells of the fetal gonad, in particular, is essential for development of a testis in males. Here we present new findings implicating a role for CaM in modulating SRY nuclear accumulation, whereby treatment of transfected cells with CaM antagonists significantly reduces nuclear accumulation of green fluorescent protein (GFP)-fusion proteins encoding either full length SRY or the SRY HMG domain alone. An in vitro nuclear transport assay using bacterially expressed fluorescent proteins showed similar results, with native gel electrophoresis/fluorimaging and fluorescence polarisation assays, indicating direct binding of CaM to the SRY HMG domain in Ca2+-dependent fashion. Since clinical mutations resulting in sex reversal occur within SRY's CaM-binding NLS, these results may shed new insight into CaM-dependent pathways of nuclear protein import, and how this may relate to testis development." @default.
- W2012081039 created "2016-06-24" @default.
- W2012081039 creator A5024701701 @default.
- W2012081039 creator A5070182251 @default.
- W2012081039 creator A5082500526 @default.
- W2012081039 creator A5084629221 @default.
- W2012081039 date "2004-01-01" @default.
- W2012081039 modified "2023-09-27" @default.
- W2012081039 title "275.Calmodulin-dependent nuclear import pathway of the testis-determining factor SRY" @default.
- W2012081039 doi "https://doi.org/10.1071/srb04abs275" @default.
- W2012081039 hasPublicationYear "2004" @default.
- W2012081039 type Work @default.
- W2012081039 sameAs 2012081039 @default.
- W2012081039 citedByCount "0" @default.
- W2012081039 crossrefType "journal-article" @default.
- W2012081039 hasAuthorship W2012081039A5024701701 @default.
- W2012081039 hasAuthorship W2012081039A5070182251 @default.
- W2012081039 hasAuthorship W2012081039A5082500526 @default.
- W2012081039 hasAuthorship W2012081039A5084629221 @default.
- W2012081039 hasBestOaLocation W20120810391 @default.
- W2012081039 hasConcept C104317684 @default.
- W2012081039 hasConcept C153911025 @default.
- W2012081039 hasConcept C190062978 @default.
- W2012081039 hasConcept C2780114586 @default.
- W2012081039 hasConcept C2780246931 @default.
- W2012081039 hasConcept C29512474 @default.
- W2012081039 hasConcept C49805395 @default.
- W2012081039 hasConcept C54355233 @default.
- W2012081039 hasConcept C59157529 @default.
- W2012081039 hasConcept C73456138 @default.
- W2012081039 hasConcept C83640560 @default.
- W2012081039 hasConcept C86339819 @default.
- W2012081039 hasConcept C86803240 @default.
- W2012081039 hasConcept C95444343 @default.
- W2012081039 hasConceptScore W2012081039C104317684 @default.
- W2012081039 hasConceptScore W2012081039C153911025 @default.
- W2012081039 hasConceptScore W2012081039C190062978 @default.
- W2012081039 hasConceptScore W2012081039C2780114586 @default.
- W2012081039 hasConceptScore W2012081039C2780246931 @default.
- W2012081039 hasConceptScore W2012081039C29512474 @default.
- W2012081039 hasConceptScore W2012081039C49805395 @default.
- W2012081039 hasConceptScore W2012081039C54355233 @default.
- W2012081039 hasConceptScore W2012081039C59157529 @default.
- W2012081039 hasConceptScore W2012081039C73456138 @default.
- W2012081039 hasConceptScore W2012081039C83640560 @default.
- W2012081039 hasConceptScore W2012081039C86339819 @default.
- W2012081039 hasConceptScore W2012081039C86803240 @default.
- W2012081039 hasConceptScore W2012081039C95444343 @default.
- W2012081039 hasIssue "9" @default.
- W2012081039 hasLocation W20120810391 @default.
- W2012081039 hasOpenAccess W2012081039 @default.
- W2012081039 hasPrimaryLocation W20120810391 @default.
- W2012081039 hasRelatedWork W1979044485 @default.
- W2012081039 hasRelatedWork W1983647029 @default.
- W2012081039 hasRelatedWork W2008897992 @default.
- W2012081039 hasRelatedWork W2012564489 @default.
- W2012081039 hasRelatedWork W2039353970 @default.
- W2012081039 hasRelatedWork W2070518772 @default.
- W2012081039 hasRelatedWork W2161134823 @default.
- W2012081039 hasRelatedWork W2168920565 @default.
- W2012081039 hasRelatedWork W2412320302 @default.
- W2012081039 hasRelatedWork W3120216242 @default.
- W2012081039 hasVolume "16" @default.
- W2012081039 isParatext "false" @default.
- W2012081039 isRetracted "false" @default.
- W2012081039 magId "2012081039" @default.
- W2012081039 workType "article" @default.