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• W2012082350 abstract "In the past two decades, as a novel approach for tissue engineering, cell sheet engineering has been proposed by our laboratory. Poly(N-isopropylacrylamide) (PIPAAm), which is a well-known temperature-responsive polymer, has been grafted on tissue culture polystyrene (TCPS) surfaces through an electron beam irradiated polymerization. At 37 °C, where the PIPAAm modified surface is hydrophobic, cells can adhere, spread on the surface and grow to confluence. By decreasing temperature to 20 °C, since the surface turns to hydrophilic, cells can detach themselves from the surface spontaneously and form an intact cell sheet with extracellular matrix. For obtaining a temperature-induced cell attachment and detachment, it is necessary to immobilize an ultra thin PIPAAm layer on the TCPS surfaces. This review focuses on the characteristics of PIAPAm modified surfaces exhibiting these intelligent properties. In addition, PIPAAm modified surfaces giving a rapid cell-sheet recovery has been further developed on the basis of the characteristic of the PIPAAm surface. The designs of temperature-responsive polymer layer have provided an enormous potential to fabricate clinically applicable regenerative medicine." @default.
• W2012082350 created "2016-06-24" @default.
• W2012082350 creator A5046565682 @default.
• W2012082350 creator A5062378145 @default.
• W2012082350 creator A5078561239 @default.
• W2012082350 date "2012-08-15" @default.
• W2012082350 modified "2023-10-07" @default.
• W2012082350 title "Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering" @default.
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• W2012082350 doi "https://doi.org/10.3390/polym4031478" @default.
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