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• W201208261 abstract "HeLa cells are widely used as a model system to study cell division. The last step of cell division, abscission, occurs at an about 1 μm wide intercellular bridge that connects the post-mitotic sister cells. Abscission often occurs long after ingression of the cleavage furrow, and no efficient methods to synchronize cells to this stage are available. Here, we have developed a correlative fluorescence time-lapse imaging and electron microscopic approach using Aclar sheets with engraved grid patterns. This grid pattern, leaving a negative imprint on thin-layer embedded samples, allows identification of cells selected from the time-lapse imaging for serial-section electron microscopy. This method facilitates the ultrastructural analysis of specific stages of abscission." @default.
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• W201208261 date "2010-01-01" @default.
• W201208261 modified "2023-10-03" @default.
• W201208261 title "Correlative Time-Lapse Imaging and Electron Microscopy to Study Abscission in HeLa Cells" @default.
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• W201208261 doi "https://doi.org/10.1016/s0091-679x(10)96024-x" @default.
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