FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012083957> ?p ?o ?g. }

• W2012083957 endingPage "e81942" @default.
• W2012083957 startingPage "e81942" @default.
• W2012083957 abstract "During a search for immunomodulatory receptors in the chicken genome, we identified a previously cloned chicken sequence as CRTAM homologue by its overall identity and several conserved sequence features. For further characterization, we generated a CRTAM specific mab. No staining was detectable in freshly isolated cell preparations from thymus, bursa, caecal tonsils, spleen, blood and intestine. Activation of splenocytes with recombinant IL-2 increased rapid CRTAM expression within a 2 h period on about 30% of the cells. These CRTAM+ cells were identified as CD8+ γδ T lymphocytes. In contrast, CRTAM expression could not be stimulated on PBL with IL-2, even within a 48 h stimulation period. As a second means of activation, T cell receptor (TCR) crosslinking using an anti-αβ-TCR induced CRTAM on both PBL and splenocytes. While CRTAM expression was again rapidly upregulated on splenocytes within 2 h, it took 48 h to reach maximum levels of CRTAM expression in PBL. Strikingly, albeit the stimulation of splenocytes was performed with anti-αβ-TCR, CRTAM expression after 2 h was mainly restricted to CD8+ γδ T lymphocytes, however, the longer anti-TCR stimulation of peripheral blood lymphocytes (PBL) resulted in CRTAM expression on αβ T lymphocytes. In order to characterize the potential ligand we cloned and expressed chicken Necl-2, a member of the nectin and nectin-like family which is highly homologous to its mammalian counterpart. Three independent assays including a reporter assay, staining with a CRTAM-Ig fusion protein and a cell conjugate assay confirmed the interaction of CRTAM with Necl-2 which could also be blocked by a soluble CRTAM-Ig fusion protein or a CRTAM specific mab. These results suggest that chicken CRTAM represents an early activation antigen on CD8+ T cells which binds to Necl-2 and is upregulated with distinct kinetics on αβ versus γδ T lymphocytes." @default.
• W2012083957 created "2016-06-24" @default.
• W2012083957 creator A5016716054 @default.
• W2012083957 creator A5017769481 @default.
• W2012083957 creator A5020648993 @default.
• W2012083957 date "2013-12-10" @default.
• W2012083957 modified "2023-09-24" @default.
• W2012083957 title "Chicken CRTAM Binds Nectin-Like 2 Ligand and Is Upregulated on CD8+ αβ and γδ T Lymphocytes with Different Kinetics" @default.
• W2012083957 cites W1484376098 @default.
• W2012083957 cites W1485925164 @default.
• W2012083957 cites W1592526725 @default.
• W2012083957 cites W1705737383 @default.
• W2012083957 cites W1771650623 @default.
• W2012083957 cites W1937114202 @default.
• W2012083957 cites W1977085566 @default.
• W2012083957 cites W1977972701 @default.
• W2012083957 cites W1985694098 @default.
• W2012083957 cites W1987704449 @default.
• W2012083957 cites W1991026243 @default.
• W2012083957 cites W2001064691 @default.
• W2012083957 cites W2020256705 @default.
• W2012083957 cites W2033467450 @default.
• W2012083957 cites W2039084448 @default.
• W2012083957 cites W2048888579 @default.
• W2012083957 cites W2051434331 @default.
• W2012083957 cites W2056624962 @default.
• W2012083957 cites W2057293799 @default.
• W2012083957 cites W2058272676 @default.
• W2012083957 cites W2058620965 @default.
• W2012083957 cites W2061142906 @default.
• W2012083957 cites W2068686330 @default.
• W2012083957 cites W2069884205 @default.
• W2012083957 cites W2072163707 @default.
• W2012083957 cites W2081836363 @default.
• W2012083957 cites W2088165672 @default.
• W2012083957 cites W2088254926 @default.
• W2012083957 cites W2091053746 @default.
• W2012083957 cites W2096482972 @default.
• W2012083957 cites W2102335230 @default.
• W2012083957 cites W2115580512 @default.
• W2012083957 cites W2118531990 @default.
• W2012083957 cites W2118538589 @default.
• W2012083957 cites W2121918046 @default.
• W2012083957 cites W2124042926 @default.
• W2012083957 cites W2132632499 @default.
• W2012083957 cites W2135449866 @default.
• W2012083957 cites W2144353489 @default.
• W2012083957 cites W2144860551 @default.
• W2012083957 cites W2149232375 @default.
• W2012083957 cites W2151862596 @default.
• W2012083957 cites W2158957141 @default.
• W2012083957 cites W2165918134 @default.
• W2012083957 cites W2166028174 @default.
• W2012083957 cites W2167333301 @default.
• W2012083957 cites W2167524807 @default.
• W2012083957 doi "https://doi.org/10.1371/journal.pone.0081942" @default.
• W2012083957 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3880370" @default.
• W2012083957 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24339981" @default.
• W2012083957 hasPublicationYear "2013" @default.
• W2012083957 type Work @default.
• W2012083957 sameAs 2012083957 @default.
• W2012083957 citedByCount "3" @default.
• W2012083957 countsByYear W20120839572016 @default.
• W2012083957 countsByYear W20120839572017 @default.
• W2012083957 countsByYear W20120839572022 @default.
• W2012083957 crossrefType "journal-article" @default.
• W2012083957 hasAuthorship W2012083957A5016716054 @default.
• W2012083957 hasAuthorship W2012083957A5017769481 @default.
• W2012083957 hasAuthorship W2012083957A5020648993 @default.
• W2012083957 hasBestOaLocation W20120839571 @default.
• W2012083957 hasConcept C114684123 @default.
• W2012083957 hasConcept C147483822 @default.
• W2012083957 hasConcept C153911025 @default.
• W2012083957 hasConcept C167672396 @default.
• W2012083957 hasConcept C170493617 @default.
• W2012083957 hasConcept C189976730 @default.
• W2012083957 hasConcept C19317047 @default.
• W2012083957 hasConcept C203014093 @default.
• W2012083957 hasConcept C2776090121 @default.
• W2012083957 hasConcept C2780931953 @default.
• W2012083957 hasConcept C55493867 @default.
• W2012083957 hasConcept C86803240 @default.
• W2012083957 hasConcept C8891405 @default.
• W2012083957 hasConceptScore W2012083957C114684123 @default.
• W2012083957 hasConceptScore W2012083957C147483822 @default.
• W2012083957 hasConceptScore W2012083957C153911025 @default.
• W2012083957 hasConceptScore W2012083957C167672396 @default.
• W2012083957 hasConceptScore W2012083957C170493617 @default.
• W2012083957 hasConceptScore W2012083957C189976730 @default.
• W2012083957 hasConceptScore W2012083957C19317047 @default.
• W2012083957 hasConceptScore W2012083957C203014093 @default.
• W2012083957 hasConceptScore W2012083957C2776090121 @default.
• W2012083957 hasConceptScore W2012083957C2780931953 @default.
• W2012083957 hasConceptScore W2012083957C55493867 @default.
• W2012083957 hasConceptScore W2012083957C86803240 @default.
• W2012083957 hasConceptScore W2012083957C8891405 @default.
• W2012083957 hasIssue "12" @default.
• W2012083957 hasLocation W20120839571 @default.

• next