FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012084881> ?p ?o ?g. }

• W2012084881 endingPage "292" @default.
• W2012084881 startingPage "286" @default.
• W2012084881 abstract "Glucose-6-phosphate dehydrogenase was purified from human placenta using DEAE-Sepharose fast flow, 2',5'-ADP Sepharose 4B column chromatography, and chromatofocusing on PBE 94 with PB 74. The enzyme was purified with 62% yield and had a specific activity of 87 units per milligram protein. The pH optimum was determined to be 7.8, using zero buffer extrapolation method. The purified placental glucose-6-phosphate dehydrogenase gave two activity bands on native PAGE: one band, constituting about 3--5% of total activity, moved slower than the remaining 95%. Among the activity bands only the faster moving band gave a band on protein staining. The slower moving band, which probably corresponded to the higher polymeric form of the G6PD with high specific activity, was not seen on native PAGE due to insufficient protein for Coomassie brilliant blue staining. The observation of one band on SDS--PAGE with an M(r) of 54 kDa and a specific activity lower than expected, suggests the presence of both forms of the G6PD, the high polymeric form at low concentration and the inactive form at high concentration, in our preparation. Measuring the activities of placental glucose-6-phosphate dehydrogenase between 20 and 50 degrees C, the activation energy, activation enthalpy, and Q(10) were calculated to be 8.16 kcal/mol, 7.55 kcal/mol, and 1.57, respectively. It was found that human placental G6PD obeys Michaelis-Menten kinetics. K(m) values were determined using the concentration ranges of 20--300 microM for G6P and 10--200 microM for NADP(+). The K(m) value for G6P was 40 microM; the K(m) value NADP(+) was found to be 20 microM. Double-reciprocal plots of 1/Vm vs 1/G6P (at constant [NADP(+)]) and of 1/Vm vs 1/NADP(+) (at constant [G6P]) intersected at the same point on the 1/V(m) axis to give V(m) = 87 U/mg protein." @default.
• W2012084881 created "2016-06-24" @default.
• W2012084881 creator A5024377829 @default.
• W2012084881 creator A5028237445 @default.
• W2012084881 creator A5059434116 @default.
• W2012084881 date "2001-03-01" @default.
• W2012084881 modified "2023-09-26" @default.
• W2012084881 title "Purification and Some Properties of Human Placental Glucose-6-Phosphate Dehydrogenase" @default.
• W2012084881 cites W106527565 @default.
• W2012084881 cites W1491720314 @default.
• W2012084881 cites W1567108311 @default.
• W2012084881 cites W1576816329 @default.
• W2012084881 cites W1592277260 @default.
• W2012084881 cites W175509376 @default.
• W2012084881 cites W196901475 @default.
• W2012084881 cites W1970018016 @default.
• W2012084881 cites W1977076271 @default.
• W2012084881 cites W1996071792 @default.
• W2012084881 cites W200857827 @default.
• W2012084881 cites W2011076813 @default.
• W2012084881 cites W2026158531 @default.
• W2012084881 cites W2044401348 @default.
• W2012084881 cites W2052932969 @default.
• W2012084881 cites W2069288455 @default.
• W2012084881 cites W2069836773 @default.
• W2012084881 cites W2079858305 @default.
• W2012084881 cites W2084135241 @default.
• W2012084881 cites W2100837269 @default.
• W2012084881 cites W2104250633 @default.
• W2012084881 cites W2111301002 @default.
• W2012084881 cites W2341758899 @default.
• W2012084881 cites W2803080115 @default.
• W2012084881 cites W4245957869 @default.
• W2012084881 cites W4293247451 @default.
• W2012084881 cites W937093307 @default.
• W2012084881 cites W964815005 @default.
• W2012084881 doi "https://doi.org/10.1006/prep.2000.1370" @default.
• W2012084881 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11237690" @default.
• W2012084881 hasPublicationYear "2001" @default.
• W2012084881 type Work @default.
• W2012084881 sameAs 2012084881 @default.
• W2012084881 citedByCount "11" @default.
• W2012084881 countsByYear W20120848812012 @default.
• W2012084881 countsByYear W20120848812013 @default.
• W2012084881 countsByYear W20120848812014 @default.
• W2012084881 countsByYear W20120848812015 @default.
• W2012084881 countsByYear W20120848812018 @default.
• W2012084881 crossrefType "journal-article" @default.
• W2012084881 hasAuthorship W2012084881A5024377829 @default.
• W2012084881 hasAuthorship W2012084881A5028237445 @default.
• W2012084881 hasAuthorship W2012084881A5059434116 @default.
• W2012084881 hasConcept C121332964 @default.
• W2012084881 hasConcept C148898269 @default.
• W2012084881 hasConcept C181199279 @default.
• W2012084881 hasConcept C185592680 @default.
• W2012084881 hasConcept C198684833 @default.
• W2012084881 hasConcept C2776317432 @default.
• W2012084881 hasConcept C2776829962 @default.
• W2012084881 hasConcept C2777132085 @default.
• W2012084881 hasConcept C2779881004 @default.
• W2012084881 hasConcept C2780196906 @default.
• W2012084881 hasConcept C2780761556 @default.
• W2012084881 hasConcept C43617362 @default.
• W2012084881 hasConcept C54355233 @default.
• W2012084881 hasConcept C55493867 @default.
• W2012084881 hasConcept C62520636 @default.
• W2012084881 hasConcept C74864618 @default.
• W2012084881 hasConcept C86803240 @default.
• W2012084881 hasConcept C96294017 @default.
• W2012084881 hasConcept C96616956 @default.
• W2012084881 hasConceptScore W2012084881C121332964 @default.
• W2012084881 hasConceptScore W2012084881C148898269 @default.
• W2012084881 hasConceptScore W2012084881C181199279 @default.
• W2012084881 hasConceptScore W2012084881C185592680 @default.
• W2012084881 hasConceptScore W2012084881C198684833 @default.
• W2012084881 hasConceptScore W2012084881C2776317432 @default.
• W2012084881 hasConceptScore W2012084881C2776829962 @default.
• W2012084881 hasConceptScore W2012084881C2777132085 @default.
• W2012084881 hasConceptScore W2012084881C2779881004 @default.
• W2012084881 hasConceptScore W2012084881C2780196906 @default.
• W2012084881 hasConceptScore W2012084881C2780761556 @default.
• W2012084881 hasConceptScore W2012084881C43617362 @default.
• W2012084881 hasConceptScore W2012084881C54355233 @default.
• W2012084881 hasConceptScore W2012084881C55493867 @default.
• W2012084881 hasConceptScore W2012084881C62520636 @default.
• W2012084881 hasConceptScore W2012084881C74864618 @default.
• W2012084881 hasConceptScore W2012084881C86803240 @default.
• W2012084881 hasConceptScore W2012084881C96294017 @default.
• W2012084881 hasConceptScore W2012084881C96616956 @default.
• W2012084881 hasIssue "2" @default.
• W2012084881 hasLocation W20120848811 @default.
• W2012084881 hasLocation W20120848812 @default.
• W2012084881 hasOpenAccess W2012084881 @default.
• W2012084881 hasPrimaryLocation W20120848811 @default.
• W2012084881 hasRelatedWork W1929969265 @default.
• W2012084881 hasRelatedWork W1970822898 @default.
• W2012084881 hasRelatedWork W1988346978 @default.
• W2012084881 hasRelatedWork W1990521089 @default.

• next