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- W2012095306 abstract "Letters to the EditorResponse to Dr. Kevin R. Short's letter to the editorBenjamin F. Miller, and Karyn L. HamiltonBenjamin F. MillerDepartment of Health and Exercise Science Colorado State University, Fort Collins, Colorado, and Karyn L. HamiltonDepartment of Health and Exercise Science Colorado State University, Fort Collins, ColoradoPublished Online:01 May 2012https://doi.org/10.1152/ajpendo.00133.2012MoreSectionsPDF (25 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat we thank dr. short (2) for taking the time to respond to our recent Perspectives article on the determination of mitochondrial biogenesis (1). We are pleased that Dr. Short has appreciated the argument we put forth for the use of mitochondrial protein synthesis as a true measure of mitochondrial biogenesis. Furthermore, we thank Dr. Short for advocating for the use of long-term measurements, as space limitations prevented us from developing this concept in the original article. Dr. Short has advocated the use of multiple methods for a “comprehensive assessment of mitochondrial function and regulation.” Of course, we agree with Dr. Short on this point. The intent of our Perspectives was not to limit these assessments but simply to more closely distinguish what “mitochondrial biogenesis” is. We believe that, although the assessment of such outcomes of mRNA content and signaling (e.g., PGC-1α) are important for the making of new mitochondria, they are not a measure of biogenesis by themselves and, in the absence of a determination of biogenesis, could lead to erroneous conclusions. Certainly, as advocated by Dr. Short, well-designed studies will include the measurement of mitochondrial biogenesis as well as the mechanisms that result in the increased rate of mitochondrial protein synthesis.DISCLOSURESNo conflicts of interest, financial or otherwise, are declared by the author(s).AUTHOR CONTRIBUTIONSAuthor contributions: B.F.M. and K.L.H. drafted manuscript; B.F.M. and K.L.H. edited and revised manuscript; B.F.M. and K.L.H. approved final version of manuscript.REFERENCES1. Miller BF , Hamilton KL . A perspective on the determination of mitochondrial biogenesis. Am J Physiol Endocrinol Metab 302: E496– E499, 2012. Link | ISI | Google Scholar2. Short KR . Measuring mitochondrial protein synthesis to assess biogenesis. Am J Physiol Endocrinol Metab; doi:10.1152/ajpendo.00117.2012. ISI | Google ScholarAUTHOR NOTESAddress for reprint requests and other correspondence: B. F. Miller, Colorado State University, 220 Moby B Complex, Fort Collins, Colorado 80523 (e-mail: Benjamin.F.[email protected]edu). Download PDF Previous Back to Top FiguresReferencesRelatedInformation CollectionsAJP-Endo CollectionsAmino Acid and Protein MetabolismExercise Related ArticlesMeasuring mitochondrial protein synthesis to assess biogenesis 01 May 2012American Journal of Physiology-Endocrinology and Metabolism More from this issue > Volume 302Issue 9May 2012Pages E1155-E1155 Copyright & PermissionsCopyright © 2012 the American Physiological Societyhttps://doi.org/10.1152/ajpendo.00133.2012History Received 14 March 2012 Accepted 14 March 2012 Published online 1 May 2012 Published in print 1 May 2012 Metrics" @default.
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