FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012097639> ?p ?o ?g. }

• W2012097639 endingPage "45" @default.
• W2012097639 startingPage "37" @default.
• W2012097639 abstract "Proteolytic processing is a key step in the production of polyphenol oxidases such as tyrosinases, converting the inactive proenzyme to an active form. In general, the fungal tyrosinase gene codes for a ~60 kDa protein that is, however, isolated as an active enzyme of ~40 kDa, lacking the C-terminal domain. Using the secreted tyrosinase 2 from Trichoderma reesei as a model protein, we performed a mutagenesis study of the residues in proximity of the experimentally determined cleavage site which are possibly involved in the proteolytic process. However, the mutant forms of tyrosinase 2 were not secreted in a full-length form retaining the C-terminal domain, but they were processed to give a ~45 kDa active form. Aiming at explaining this phenomenon, we analysed in silico the properties of the C-terminal domain of tyrosinase 2, of 23 previously retrieved homologous tyrosinase sequences from fungi (C. Gasparetti, G. Faccio, M. Arvas, J. Buchert, M. Saloheimo, K. Kruus, Appl. Microbiol. Biotechnol. 86 (2010) 213-226) and of nine well-characterised polyphenol oxidases. Based on the results of our study, we exclude the key role of specific amino acids at the cleavage site in the proteolytic process and report an overall higher sensitivity to proteolysis of the linker region and of the whole C-terminal domain of fungal tyrosinases." @default.
• W2012097639 created "2016-06-24" @default.
• W2012097639 creator A5009032164 @default.
• W2012097639 creator A5036297981 @default.
• W2012097639 creator A5056489686 @default.
• W2012097639 creator A5068151631 @default.
• W2012097639 date "2013-04-01" @default.
• W2012097639 modified "2023-10-03" @default.
• W2012097639 title "Experimental and bioinformatic investigation of the proteolytic degradation of the C-terminal domain of a fungal tyrosinase" @default.
• W2012097639 cites W1535193939 @default.
• W2012097639 cites W1579900246 @default.
• W2012097639 cites W1613863775 @default.
• W2012097639 cites W1620636612 @default.
• W2012097639 cites W1686801194 @default.
• W2012097639 cites W1755255420 @default.
• W2012097639 cites W1965249399 @default.
• W2012097639 cites W1967478628 @default.
• W2012097639 cites W1971218701 @default.
• W2012097639 cites W1973127724 @default.
• W2012097639 cites W1986646814 @default.
• W2012097639 cites W1988888691 @default.
• W2012097639 cites W1991275535 @default.
• W2012097639 cites W1996423252 @default.
• W2012097639 cites W1998675895 @default.
• W2012097639 cites W2001449241 @default.
• W2012097639 cites W2006192061 @default.
• W2012097639 cites W2007602225 @default.
• W2012097639 cites W2010601153 @default.
• W2012097639 cites W2011901860 @default.
• W2012097639 cites W2014179578 @default.
• W2012097639 cites W2019214848 @default.
• W2012097639 cites W2019614811 @default.
• W2012097639 cites W2024563032 @default.
• W2012097639 cites W2026594509 @default.
• W2012097639 cites W2027440025 @default.
• W2012097639 cites W2031436925 @default.
• W2012097639 cites W2031586773 @default.
• W2012097639 cites W2041056624 @default.
• W2012097639 cites W2042617105 @default.
• W2012097639 cites W2047602616 @default.
• W2012097639 cites W2052002049 @default.
• W2012097639 cites W2052562948 @default.
• W2012097639 cites W2052565572 @default.
• W2012097639 cites W2053820259 @default.
• W2012097639 cites W2057021995 @default.
• W2012097639 cites W2060548396 @default.
• W2012097639 cites W2060913542 @default.
• W2012097639 cites W2062489857 @default.
• W2012097639 cites W2066493478 @default.
• W2012097639 cites W2067615149 @default.
• W2012097639 cites W2069104797 @default.
• W2012097639 cites W2072648459 @default.
• W2012097639 cites W2073681519 @default.
• W2012097639 cites W2081418090 @default.
• W2012097639 cites W2082528610 @default.
• W2012097639 cites W2083571924 @default.
• W2012097639 cites W2083903819 @default.
• W2012097639 cites W2084475928 @default.
• W2012097639 cites W2089047063 @default.
• W2012097639 cites W2090962336 @default.
• W2012097639 cites W2091706492 @default.
• W2012097639 cites W2094435333 @default.
• W2012097639 cites W2102455520 @default.
• W2012097639 cites W2102818658 @default.
• W2012097639 cites W2106276549 @default.
• W2012097639 cites W2106515998 @default.
• W2012097639 cites W2108640886 @default.
• W2012097639 cites W2120772351 @default.
• W2012097639 cites W2125637425 @default.
• W2012097639 cites W2135143214 @default.
• W2012097639 cites W2137015675 @default.
• W2012097639 cites W2137450588 @default.
• W2012097639 cites W2138006485 @default.
• W2012097639 cites W2146967756 @default.
• W2012097639 cites W2148920429 @default.
• W2012097639 cites W2158113006 @default.
• W2012097639 cites W2161036854 @default.
• W2012097639 cites W2162798426 @default.
• W2012097639 doi "https://doi.org/10.1016/j.jinorgbio.2012.12.006" @default.
• W2012097639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23333757" @default.
• W2012097639 hasPublicationYear "2013" @default.
• W2012097639 type Work @default.
• W2012097639 sameAs 2012097639 @default.
• W2012097639 citedByCount "20" @default.
• W2012097639 countsByYear W20120976392014 @default.
• W2012097639 countsByYear W20120976392015 @default.
• W2012097639 countsByYear W20120976392016 @default.
• W2012097639 countsByYear W20120976392017 @default.
• W2012097639 countsByYear W20120976392018 @default.
• W2012097639 countsByYear W20120976392019 @default.
• W2012097639 countsByYear W20120976392020 @default.
• W2012097639 countsByYear W20120976392021 @default.
• W2012097639 countsByYear W20120976392022 @default.
• W2012097639 countsByYear W20120976392023 @default.
• W2012097639 crossrefType "journal-article" @default.
• W2012097639 hasAuthorship W2012097639A5009032164 @default.
• W2012097639 hasAuthorship W2012097639A5036297981 @default.
• W2012097639 hasAuthorship W2012097639A5056489686 @default.

• next