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- W2012105719 abstract "Summary Cryopreservation experiments were performed using ginsenoside-producing cell cultures of Panax ginseng. We obtained viable cells after storage at the temperature of liquid nitrogen. The protocol was optimised with regard to preeculture conditions and cryoprotectants. The relatively low freezing tolerance of ginseng cells was enhanced from less than 10 % to about 40 % as expressed by cell viability immediately after thawing. Though less than 50 % of the cell population survived, no selection occurred. The growth patterns of frozen-thawed and control cultures were identical. This is shown on the basis of fresh and dry weight, cell number, and packed cell volume. In addition, frozen-thawed ginseng cultures were shown to retain their capacity for producing ginsenosides. Using HPLC analysis the following components were determined: ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, and Rd. Neither ginsenoside yield nor ginsenoside pattern were influenced by cryostorage." @default.
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- W2012105719 date "1987-12-01" @default.
- W2012105719 modified "2023-09-24" @default.
- W2012105719 title "Growth and Ginsenoside Patterns of Cryopreserved Panax ginseng Cell Cultures" @default.
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- W2012105719 doi "https://doi.org/10.1016/s0176-1617(87)80161-x" @default.
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