Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012106066> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2012106066 endingPage "181" @default.
- W2012106066 startingPage "181" @default.
- W2012106066 abstract "Bovine growth hormone (bGH) has been used to improve the results for in vitro production of bovine embryos. Inclusion of bGH in the maturation medium increases both rate of cleavage and frequency of blastocyst development. Thus, the purpose of the present study was to evaluate the effect of recombinant bovine somatotropin (rBST) on cleavage and blastocyst development of bovine embryos when included during in vitro maturation (IVM) only (Group 1), during both IVM and in vitro culture (IVC; Group 2), during IVC only (Group 3), or not included during either IVM or IVC (Group 4). Specifically, in Group 1, oocytes were matured in TCM 199 (Earle's salts) supplemented with 10% FCS, LH, FSH, oestradiol, and amikacin (IVM medium), plus 100 ng mL–1 of rBST and cultured in SOFaaci supplemented with essential amino acids, tri-sodium citrate, myo-inositol, and 5% FBS. In group 2, oocytes were matured in IVM medium containing 100 ng mL–1 of rBST and cultured in SOFaaci supplemented with essential amino acid, tri-sodium citrate, myo-inositol, 5% FBS; on Day 5, rBST (50 ng mL–1) was added. In Group 3, oocytes were matured in IVM medium without rBST; on Day 5, rBST (50 ng mL–1) was added. Group 4 was the control, without rBST supplementation. The treatment groups were analysed using the SAS® (SAS Institute Inc., Cary, NC, USA) in a completely randomised design (P < 0.05). Somatotropin has receptors in cumulus cells and in the zona pellucida acting directly in the oocyte; however, the increase in cleavage rate seen in previous studies after rBST treatment was not observed in the present study. Supplementation of culture medium with rBST during Day 2 to 6 of IVC has been shown to increase the number of trophoblast and subsequent pregnancy rate following transfer. However, in the present study, addition of 50 and 100 ng mL–1 of rBST to the maturation or culture medium did not affect the cleavage rate of embryos and blastocyst production. Table 1.Analysis of the meaning and percentages related to cleavage and production of embryos" @default.
- W2012106066 created "2016-06-24" @default.
- W2012106066 creator A5013520474 @default.
- W2012106066 creator A5018676720 @default.
- W2012106066 creator A5048703292 @default.
- W2012106066 creator A5058532103 @default.
- W2012106066 creator A5076975294 @default.
- W2012106066 creator A5083292300 @default.
- W2012106066 date "2014-01-01" @default.
- W2012106066 modified "2023-09-26" @default.
- W2012106066 title "135 RECOMBINANT BOVINE SOMATOTROPIN ON THE IN VITRO PRODUCTION OF BOVINE EMBRYOS" @default.
- W2012106066 doi "https://doi.org/10.1071/rdv26n1ab135" @default.
- W2012106066 hasPublicationYear "2014" @default.
- W2012106066 type Work @default.
- W2012106066 sameAs 2012106066 @default.
- W2012106066 citedByCount "0" @default.
- W2012106066 crossrefType "journal-article" @default.
- W2012106066 hasAuthorship W2012106066A5013520474 @default.
- W2012106066 hasAuthorship W2012106066A5018676720 @default.
- W2012106066 hasAuthorship W2012106066A5048703292 @default.
- W2012106066 hasAuthorship W2012106066A5058532103 @default.
- W2012106066 hasAuthorship W2012106066A5076975294 @default.
- W2012106066 hasAuthorship W2012106066A5083292300 @default.
- W2012106066 hasConcept C104317684 @default.
- W2012106066 hasConcept C16685009 @default.
- W2012106066 hasConcept C177752829 @default.
- W2012106066 hasConcept C179933525 @default.
- W2012106066 hasConcept C185592680 @default.
- W2012106066 hasConcept C196843134 @default.
- W2012106066 hasConcept C202751555 @default.
- W2012106066 hasConcept C2775966511 @default.
- W2012106066 hasConcept C2776690073 @default.
- W2012106066 hasConcept C2777324261 @default.
- W2012106066 hasConcept C2778177303 @default.
- W2012106066 hasConcept C2779335785 @default.
- W2012106066 hasConcept C2984496839 @default.
- W2012106066 hasConcept C55493867 @default.
- W2012106066 hasConcept C71315377 @default.
- W2012106066 hasConcept C71924100 @default.
- W2012106066 hasConcept C86803240 @default.
- W2012106066 hasConcept C87073359 @default.
- W2012106066 hasConcept C95444343 @default.
- W2012106066 hasConceptScore W2012106066C104317684 @default.
- W2012106066 hasConceptScore W2012106066C16685009 @default.
- W2012106066 hasConceptScore W2012106066C177752829 @default.
- W2012106066 hasConceptScore W2012106066C179933525 @default.
- W2012106066 hasConceptScore W2012106066C185592680 @default.
- W2012106066 hasConceptScore W2012106066C196843134 @default.
- W2012106066 hasConceptScore W2012106066C202751555 @default.
- W2012106066 hasConceptScore W2012106066C2775966511 @default.
- W2012106066 hasConceptScore W2012106066C2776690073 @default.
- W2012106066 hasConceptScore W2012106066C2777324261 @default.
- W2012106066 hasConceptScore W2012106066C2778177303 @default.
- W2012106066 hasConceptScore W2012106066C2779335785 @default.
- W2012106066 hasConceptScore W2012106066C2984496839 @default.
- W2012106066 hasConceptScore W2012106066C55493867 @default.
- W2012106066 hasConceptScore W2012106066C71315377 @default.
- W2012106066 hasConceptScore W2012106066C71924100 @default.
- W2012106066 hasConceptScore W2012106066C86803240 @default.
- W2012106066 hasConceptScore W2012106066C87073359 @default.
- W2012106066 hasConceptScore W2012106066C95444343 @default.
- W2012106066 hasIssue "1" @default.
- W2012106066 hasLocation W20121060661 @default.
- W2012106066 hasOpenAccess W2012106066 @default.
- W2012106066 hasPrimaryLocation W20121060661 @default.
- W2012106066 hasRelatedWork W1970819264 @default.
- W2012106066 hasRelatedWork W1991072146 @default.
- W2012106066 hasRelatedWork W1996665414 @default.
- W2012106066 hasRelatedWork W2006214884 @default.
- W2012106066 hasRelatedWork W2032379651 @default.
- W2012106066 hasRelatedWork W2072434133 @default.
- W2012106066 hasRelatedWork W2183933865 @default.
- W2012106066 hasRelatedWork W2885382957 @default.
- W2012106066 hasRelatedWork W2990953945 @default.
- W2012106066 hasRelatedWork W4312129045 @default.
- W2012106066 hasVolume "26" @default.
- W2012106066 isParatext "false" @default.
- W2012106066 isRetracted "false" @default.
- W2012106066 magId "2012106066" @default.
- W2012106066 workType "article" @default.