Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012110555> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2012110555 endingPage "2615" @default.
- W2012110555 startingPage "2609" @default.
- W2012110555 abstract "The respiratory burst of human polymorphonuclear neutrophils (PMN) induced by particle or soluble stimuli was measured in the presence of the nonsteroidal anti-inflammatory drug, diclofenac sodium (Voltaren). Diclofenac (25-100 micrograms/ml) inhibited the oxygen consumption of PMN stimulated by 5 X 10(-7) M of N-formyl-methionyl-leucyl-phenylalanine (FMLP). The inhibition was linearly correlated to diclofenac concentration. By contrast, diclofenac did not affect the rate of heat-killed Klebsiella pneumoniae ingestion of PMN, or the PMN O2-uptake induced by (0.67 microgram/ml) serum-opsonized zymosan or (1 microgram/ml) phorbol myristate acetate (PMA). The PMN production of superoxide anion induced by various FMLP concentrations (10(-7), 10(-6) and 10(-5) M) was also decreased by diclofenac. However, this inhibition declined when the formylated peptide concentration was raised suggesting that diclofenac could alter FMLP binding to the PMN membrane. Binding experiments of tritiated FMLP to intact PMN performed at 22 degrees and 4 degrees showed high- and low-affinity FMLP sites with dissociation constant (Kd) values of approximately 2 X 10(-8) M and 10(-5) M respectively. Diclofenac did not significantly alter the low-affinity component but induced modifications of the high-affinity component which were different at 22 degrees and 4 degrees. At 22 degrees only the dissociation constant value was enhanced by diclofenac (competitive inhibition) whereas at 4 degrees both binding parameters (i.e. dissociation constant and number of available binding sites) were modified (mixed inhibition). Diclofenac was also shown to bind to PMN with a low affinity. This binding was not diminished at 4 degrees by various concentrations of FMLP which even increased the number of diclofenac binding sites on PMN at 22 degrees. These data suggest that diclofenac binding to PMN may decrease FMLP-induced PMN respiratory burst by interfering with the peptide recognition by specific FMLP receptors." @default.
- W2012110555 created "2016-06-24" @default.
- W2012110555 creator A5016365800 @default.
- W2012110555 creator A5037011842 @default.
- W2012110555 creator A5055188623 @default.
- W2012110555 creator A5070787008 @default.
- W2012110555 date "1987-08-01" @default.
- W2012110555 modified "2023-10-17" @default.
- W2012110555 title "Diclofenac binding to human polymorphonuclear neutrophils: Effect on respiratory burst and N-formylated peptide binding" @default.
- W2012110555 cites W13249423 @default.
- W2012110555 cites W1503509391 @default.
- W2012110555 cites W156904301 @default.
- W2012110555 cites W1679156369 @default.
- W2012110555 cites W1964189456 @default.
- W2012110555 cites W1964741048 @default.
- W2012110555 cites W1965340455 @default.
- W2012110555 cites W1971090056 @default.
- W2012110555 cites W1974556122 @default.
- W2012110555 cites W1983649348 @default.
- W2012110555 cites W1989895978 @default.
- W2012110555 cites W1991776975 @default.
- W2012110555 cites W2013557847 @default.
- W2012110555 cites W2016802338 @default.
- W2012110555 cites W2018270860 @default.
- W2012110555 cites W2020704694 @default.
- W2012110555 cites W2031103039 @default.
- W2012110555 cites W2043251609 @default.
- W2012110555 cites W2043813981 @default.
- W2012110555 cites W2044436395 @default.
- W2012110555 cites W2053702981 @default.
- W2012110555 cites W2080662368 @default.
- W2012110555 cites W2091934678 @default.
- W2012110555 cites W2172019543 @default.
- W2012110555 cites W2274125221 @default.
- W2012110555 cites W2415037994 @default.
- W2012110555 cites W2418974924 @default.
- W2012110555 doi "https://doi.org/10.1016/0006-2952(87)90539-9" @default.
- W2012110555 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3300657" @default.
- W2012110555 hasPublicationYear "1987" @default.
- W2012110555 type Work @default.
- W2012110555 sameAs 2012110555 @default.
- W2012110555 citedByCount "25" @default.
- W2012110555 countsByYear W20121105552021 @default.
- W2012110555 crossrefType "journal-article" @default.
- W2012110555 hasAuthorship W2012110555A5016365800 @default.
- W2012110555 hasAuthorship W2012110555A5037011842 @default.
- W2012110555 hasAuthorship W2012110555A5055188623 @default.
- W2012110555 hasAuthorship W2012110555A5070787008 @default.
- W2012110555 hasConcept C170493617 @default.
- W2012110555 hasConcept C181199279 @default.
- W2012110555 hasConcept C185592680 @default.
- W2012110555 hasConcept C2779281246 @default.
- W2012110555 hasConcept C2779826273 @default.
- W2012110555 hasConcept C2780795997 @default.
- W2012110555 hasConcept C43617362 @default.
- W2012110555 hasConcept C55493867 @default.
- W2012110555 hasConcept C74998103 @default.
- W2012110555 hasConcept C99971728 @default.
- W2012110555 hasConceptScore W2012110555C170493617 @default.
- W2012110555 hasConceptScore W2012110555C181199279 @default.
- W2012110555 hasConceptScore W2012110555C185592680 @default.
- W2012110555 hasConceptScore W2012110555C2779281246 @default.
- W2012110555 hasConceptScore W2012110555C2779826273 @default.
- W2012110555 hasConceptScore W2012110555C2780795997 @default.
- W2012110555 hasConceptScore W2012110555C43617362 @default.
- W2012110555 hasConceptScore W2012110555C55493867 @default.
- W2012110555 hasConceptScore W2012110555C74998103 @default.
- W2012110555 hasConceptScore W2012110555C99971728 @default.
- W2012110555 hasIssue "16" @default.
- W2012110555 hasLocation W20121105551 @default.
- W2012110555 hasLocation W20121105552 @default.
- W2012110555 hasOpenAccess W2012110555 @default.
- W2012110555 hasPrimaryLocation W20121105551 @default.
- W2012110555 hasRelatedWork W1482519158 @default.
- W2012110555 hasRelatedWork W2009599121 @default.
- W2012110555 hasRelatedWork W2012110555 @default.
- W2012110555 hasRelatedWork W2025582096 @default.
- W2012110555 hasRelatedWork W2063340476 @default.
- W2012110555 hasRelatedWork W2072992117 @default.
- W2012110555 hasRelatedWork W2074767739 @default.
- W2012110555 hasRelatedWork W2079019468 @default.
- W2012110555 hasRelatedWork W2419327616 @default.
- W2012110555 hasRelatedWork W2466721288 @default.
- W2012110555 hasVolume "36" @default.
- W2012110555 isParatext "false" @default.
- W2012110555 isRetracted "false" @default.
- W2012110555 magId "2012110555" @default.
- W2012110555 workType "article" @default.