FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012112711> ?p ?o ?g. }

• W2012112711 endingPage "2935" @default.
• W2012112711 startingPage "2928" @default.
• W2012112711 abstract "ABSTRACT The Klebsiella oxytoca pullulanase secreton (type II secretion system) components PulM and PulL were tagged at their N termini with green fluorescent protein (GFP), and their subcellular location was examined by fluorescence microscopy and fractionation. When produced at moderate levels without other secreton components in Escherichia coli , both chimeras were envelope associated, as are the native proteins. Fluorescent GFP-PulM was evenly distributed over the cell envelope, with occasional brighter foci. Under the same conditions, GFP-PulL was barely detectable in the envelope by fluorescence microscopy. When produced together with all other secreton components, GFP-PulL exhibited circumferential fluorescence, with numerous brighter patches. The envelope-associated fluorescence of GFP-PulL was almost completely abolished when native PulL was also produced, suggesting that the chimera cannot compete with PulL for association with other secreton components. The patches of GFP-PulL might represent functional secretons, since GFP-PulM also appeared in similar patches. GFP-PulM and GFP-PulL both appeared in spherical polar foci when made at high levels. In K. oxytoca , GFP-PulM was evenly distributed over the cell envelope, with few patches, whereas GFP-PulL showed only weak envelope-associated fluorescence. These data suggest that, in contrast to their Vibrio cholerae Eps secreton counterparts (M. Scott, Z. Dossani, and M. Sandkvist, Proc. Natl. Acad. Sci. USA 98:13978-13983, 2001), PulM and PulL do not localize specifically to the cell poles and that the Pul secreton is distributed over the cell surface." @default.
• W2012112711 created "2016-06-24" @default.
• W2012112711 creator A5007456673 @default.
• W2012112711 creator A5084528185 @default.
• W2012112711 creator A5084713719 @default.
• W2012112711 date "2006-04-15" @default.
• W2012112711 modified "2023-10-03" @default.
• W2012112711 title "Green Fluorescent Chimeras Indicate Nonpolar Localization of Pullulanase Secreton Components PulL and PulM" @default.
• W2012112711 cites W111835664 @default.
• W2012112711 cites W1483391222 @default.
• W2012112711 cites W1599414603 @default.
• W2012112711 cites W1847448852 @default.
• W2012112711 cites W1899289205 @default.
• W2012112711 cites W1914922901 @default.
• W2012112711 cites W1932645010 @default.
• W2012112711 cites W1969886111 @default.
• W2012112711 cites W1970930084 @default.
• W2012112711 cites W1972796096 @default.
• W2012112711 cites W1973770205 @default.
• W2012112711 cites W1979581724 @default.
• W2012112711 cites W1983311630 @default.
• W2012112711 cites W1984691730 @default.
• W2012112711 cites W1985079532 @default.
• W2012112711 cites W1986663500 @default.
• W2012112711 cites W1989302103 @default.
• W2012112711 cites W1992003720 @default.
• W2012112711 cites W2007402269 @default.
• W2012112711 cites W2008148610 @default.
• W2012112711 cites W2026174297 @default.
• W2012112711 cites W2029527367 @default.
• W2012112711 cites W2033941975 @default.
• W2012112711 cites W2036445018 @default.
• W2012112711 cites W2052935507 @default.
• W2012112711 cites W2061051066 @default.
• W2012112711 cites W2069622113 @default.
• W2012112711 cites W2081260914 @default.
• W2012112711 cites W2087631877 @default.
• W2012112711 cites W2096366905 @default.
• W2012112711 cites W2097464195 @default.
• W2012112711 cites W2102067798 @default.
• W2012112711 cites W2104351524 @default.
• W2012112711 cites W2104744489 @default.
• W2012112711 cites W2104878600 @default.
• W2012112711 cites W2111048966 @default.
• W2012112711 cites W2112802037 @default.
• W2012112711 cites W2119998174 @default.
• W2012112711 cites W2123440179 @default.
• W2012112711 cites W2132929413 @default.
• W2012112711 cites W2140237288 @default.
• W2012112711 cites W2148612003 @default.
• W2012112711 cites W2148846022 @default.
• W2012112711 cites W2150081979 @default.
• W2012112711 cites W2153386849 @default.
• W2012112711 cites W2153775455 @default.
• W2012112711 cites W2154214883 @default.
• W2012112711 cites W2156489423 @default.
• W2012112711 cites W2162876821 @default.
• W2012112711 cites W2165059275 @default.
• W2012112711 cites W2168891068 @default.
• W2012112711 cites W2169420537 @default.
• W2012112711 doi "https://doi.org/10.1128/jb.188.8.2928-2935.2006" @default.
• W2012112711 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1447010" @default.
• W2012112711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16585754" @default.
• W2012112711 hasPublicationYear "2006" @default.
• W2012112711 type Work @default.
• W2012112711 sameAs 2012112711 @default.
• W2012112711 citedByCount "24" @default.
• W2012112711 countsByYear W20121127112012 @default.
• W2012112711 countsByYear W20121127112013 @default.
• W2012112711 countsByYear W20121127112014 @default.
• W2012112711 countsByYear W20121127112016 @default.
• W2012112711 countsByYear W20121127112017 @default.
• W2012112711 countsByYear W20121127112022 @default.
• W2012112711 countsByYear W20121127112023 @default.
• W2012112711 crossrefType "journal-article" @default.
• W2012112711 hasAuthorship W2012112711A5007456673 @default.
• W2012112711 hasAuthorship W2012112711A5084528185 @default.
• W2012112711 hasAuthorship W2012112711A5084713719 @default.
• W2012112711 hasBestOaLocation W20121127111 @default.
• W2012112711 hasConcept C104317684 @default.
• W2012112711 hasConcept C121332964 @default.
• W2012112711 hasConcept C12554922 @default.
• W2012112711 hasConcept C142613039 @default.
• W2012112711 hasConcept C169274487 @default.
• W2012112711 hasConcept C181199279 @default.
• W2012112711 hasConcept C2779423947 @default.
• W2012112711 hasConcept C55493867 @default.
• W2012112711 hasConcept C62520636 @default.
• W2012112711 hasConcept C86803240 @default.
• W2012112711 hasConcept C91881484 @default.
• W2012112711 hasConcept C95444343 @default.
• W2012112711 hasConceptScore W2012112711C104317684 @default.
• W2012112711 hasConceptScore W2012112711C121332964 @default.
• W2012112711 hasConceptScore W2012112711C12554922 @default.
• W2012112711 hasConceptScore W2012112711C142613039 @default.
• W2012112711 hasConceptScore W2012112711C169274487 @default.
• W2012112711 hasConceptScore W2012112711C181199279 @default.
• W2012112711 hasConceptScore W2012112711C2779423947 @default.

• next