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- W2012116211 abstract "Introduction: TSP-1 up-regulates MMP-9, a key protease in tumor cell invasion, in gastric cancer. We have also shown that angiocidin, a novel TSP-1 receptor, co-localizes with MMP-9 in gastric cancer. We hypothesize that angiocidin plays a central role in TSP-1 up-regulation of MMP-9 in gastric cancer cells. Methods: AGS gastric adenocarcinoma cells were incubated with exogenous TSP-1 or co-cultured with TSP-1-producing NHDF fibroblasts. TSP-1 expression was determined by ELISA and Western blot. The effect of TSP-1 on MMP-9 expression was determined by using anti-TSP-1 or anti-angiocidin antibodies. MMP-9 expression was determined by Western blot, zymography, and quantitative immunohistochemistry. Statistical significance was determined by ANOVA and Student’s t-test. Results: ELISA and Western blot showed a 20-fold difference in TSP-1 expression between NHDF and AGS cells (100 vs. 5 g/106 cells/24 hours of TSP-1, respectively, p < 0.01). Exogenous TSP-1 up-regulated MMP-9 production by AGS cells twofold (see Table and Fig.). TABLE—ABSTRACT 95MMP-9 Expression by Quantitative Immunohistochemistry Treatment group AGS AGS + TSP-1 ASG + NHDF ASG + NHDF + anti-TSP-1 AGS/NHDF + anti-angiocidin Cell No. 13 23 ∗ P < 0.01 vs. AGS. 121 24 P < 0.01 vs. AGS + NHDF. 15 P < 0.01 vs. AGS + NHDF. Numbers represent number of MMP-9-positive AGS cells per high power field. ∗ P < 0.01 vs. AGS. ∗∗ P < 0.01 vs. AGS + NHDF. Open table in a new tab Co-culturing AGS cells with TSP-1-producing NHDF fibroblasts up-regulated AGS cell MMP-9 expression tenfold (see Table and Fig.). MMP-9 expression was reduced 80% and 90% by anti-TSP-1 or anti-angiocidin antibodies, respectively (see Table and Fig.). Conclusions: We conclude that TSP-1, through angiocidin, up-regulates MMP-9 expression in gastric carcinoma cells. This effect is more prominent with tumor cell-stromal cell interaction. Graphical Abstract View Large Image Figure Viewer Download Hi-res image" @default.
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- W2012116211 date "2003-10-01" @default.
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- W2012116211 title "Thrombospondin-1 (TSP-1) up-regulates matrix metalloproteinase-9 (MMP-9) expression through a novel receptor in gastric cancer cells" @default.
- W2012116211 doi "https://doi.org/10.1016/j.jss.2003.08.221" @default.
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