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- W2012119908 abstract "An enzymatic determination method for galactosylceramide galactosidase (EC 3.2.1.46) was devised by using an enzymatic amplification reaction, NAD cycling. Galactose released by crude enzyme samples (tissue homogenates and cell suspensions) from galactosylceramide quantitatively reduced NAD to NADH by the galactose dehydrogenase reaction; then the NADH was amplified 6000-10,000-fold by NAD cycling and determined fluorometrically. A higher sensitivity of assay was obtained compared with the previous radiometric method. The present method was successfully applied to tissues from patients with Krabbe's disease, whose organs are deficient in galactosidase. The galactosidase reaction rate with a crude sample was not proportional to its concentration. However, the double-reciprocal plot of the reaction rate against the sample concentration became linear and provided a unique value of specific activity to each sample." @default.
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- W2012119908 date "1982-10-01" @default.
- W2012119908 modified "2023-10-03" @default.
- W2012119908 title "Enzymatic determination of galactosylceramide galactosidase in tissues by NAD cycling" @default.
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- W2012119908 doi "https://doi.org/10.1016/0003-2697(82)90106-3" @default.
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