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- W2012121544 abstract "ABSTRACT Pseudomonas putida KT2442 is a root-colonizing strain which can use proline, one of the major components in root exudates, as its sole carbon and nitrogen source. A P. putida mutant unable to grow with proline as the sole carbon and nitrogen source was isolated after random mini-Tn 5 –Km mutagenesis. The mini-Tn 5 insertion was located at the putA gene, which is adjacent to and divergent from the putP gene. The putA gene codes for a protein of 1,315 amino acid residues which is homologous to the PutA protein of Escherichia coli , Salmonella enterica serovar Typhimurium, Rhodobacter capsulatus , and several Rhizobium strains. The central part of P. putida PutA showed homology to the proline dehydrogenase of Saccharomyces cerevisiae and Drosophila melanogaster , whereas the C-terminal end was homologous to the pyrroline-5-carboxylate dehydrogenase of S. cerevisiae and a number of aldehyde dehydrogenases. This suggests that in P. putida , both enzymatic steps for proline conversion to glutamic acid are catalyzed by a single polypeptide. The putP gene was homologous to the putP genes of several prokaryotic microorganisms, and its gene product is an integral inner-membrane protein involved in the uptake of proline. The expression of both genes was induced by proline added in the culture medium and was regulated by PutA. In a P. putida putA -deficient background, expression of both putA and putP genes was maximal and proline independent. Corn root exudates collected during 7 days also strongly induced the P. putida put genes, as determined by using fusions of the put promoters to ′ lacZ . The induction ratio for the putA promoter (about 20-fold) was 6-fold higher than the induction ratio for the putP promoter." @default.
- W2012121544 created "2016-06-24" @default.
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- W2012121544 date "2000-01-01" @default.
- W2012121544 modified "2023-10-18" @default.
- W2012121544 title "Proline Catabolism by <i>Pseudomonas putida</i> : Cloning, Characterization, and Expression of the <i>put</i> Genes in the Presence of Root Exudates" @default.
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- W2012121544 doi "https://doi.org/10.1128/jb.182.1.91-99.2000" @default.
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