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- W2012122516 abstract "Cadmium (Cd) is a well-known industrial and environmental pollutant that can induce cell death in root tip cells of Allium cepa Linnaeus. Cell death is induced by two modes: apoptosis and necrosis. We used a TUNEL assay and DNA gel electrophoresis on Cd-treated cells of onion root to morphologically distinguish the mode of cell death. Spheres of condensed chromatin, a condensed and marginal nucleus, fragmented DNA, and formed apoptotic bodies, all characteristics of apoptotic death, were observed in medium concentrations of Cd (25–50 μM). TUNEL-positive cells were observed in cortex and pericycle cells of root tips after exposure to 20 μM Cd. At higher doses (50 μM), TUNEL-positive cells were also observed in the meristem. At 25–50 μM Cd, the ladders of the oligonucleosomal DNA fragments were detected on 2% agarose gel combined with detection of apoptotic bodies by TUNEL assay. Therefore, Cd induction of apoptosis is dose-dependent; above a threshold level of 25–50 μM, cells experience necrosis which leads to cell lysis and leakage." @default.
- W2012122516 created "2016-06-24" @default.
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- W2012122516 date "2004-09-01" @default.
- W2012122516 modified "2023-10-15" @default.
- W2012122516 title "Detection of apoptotic bodies and oligonucleosomal DNA fragments in cadmium-treated root apical cells of Allium cepa Linnaeus" @default.
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- W2012122516 doi "https://doi.org/10.1016/j.plantsci.2004.04.024" @default.
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