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• W2012122858 abstract "Compact triphenylphosphine oxide or tripiperidinophosphine oxide films formed at Hg surfaces are shown to increase Cd, Zn-metal-lothionenin (MT) adsorptivity at the polarized electrode/solution interface. Interactions of the polar P=O group of these adsorbates with the hydrophilic centers located on the metalloprotein exterior are suggested as being responsible for this phenomenon. The resulting cathodic current (ic) due to the reduction of coordinated Cd(II) in Cd,Zn-MT increases more than seven times in the presence of 1.0 × 10−3 M triphenylphosphine oxide. The ic response at about −0.72 V vs. Ag/AgCl then varies linearly with the bulk concentration of Cd,Zn-MT from 2 × 10−9 to 5 × 10−5 mol dm−3. Detection limit for the rabbit liver Cd,Zn-MT is found to be 8.9 × 10−10 mol dm−3 using the differential pulse voltammetric technique on a hanging mercury drop electrode. This novel method for the detection of the thiolate-chelated Cd(II) complexes allows the filtering out of cathodic currents due to electroreduction of the free-uncoordinated Cd2+ ions which might interfere with the MT determination in some biological samples. Advantages of the present method compared to other electroanalytical methods for MT determination are discussed." @default.
• W2012122858 created "2016-06-24" @default.
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• W2012122858 date "1996-08-01" @default.
• W2012122858 modified "2023-09-25" @default.
• W2012122858 title "Adsorption of Cd,Zn-metallothionein on covered Hg electrodes and its voltammetric determination" @default.
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• W2012122858 doi "https://doi.org/10.1016/0302-4598(96)01914-9" @default.
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