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- W2012123983 abstract "Cellulose production from sucrose by Acetobacter strains is accompanied by the accumulation of a water-soluble polysaccharide, called levan. To improve cellulose productivity, a levansucrase-deficient mutant, LD-2, was derived from Acetobacter strain 757 and used as a host for the construction of recombinant strains. An LD-2 mutant harboring a plasmid containing the sucrase gene, sucZE3, from Zymomonas mobilis together with zliS, a gene that encodes a secretion-activating factor under the control of the Escherichia coli lac promoter, had sucrase activity and produced much cellulose and little levan in a medium containing sucrose. In addition, a mutant levansucrase gene, mutant sacB, from Bacillus subtilis, which encodes a protein with little levan-forming activity, was generated by site-directed mutagenesis and introduced into the LD-2 mutant. This introduction also resulted in the higher cellulose productivity and little levan." @default.
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- W2012123983 date "1998-01-01" @default.
- W2012123983 modified "2023-10-03" @default.
- W2012123983 title "Increased Cellulose Production from Sucrose with Reduced Levan Accumulation by anAcetobacterStrain Harboring a Recombinant Plasmid" @default.
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- W2012123983 doi "https://doi.org/10.1271/bbb.62.833" @default.
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