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- W2012128325 abstract "We present Monte Carlo simulations of the self-assembly of bivalent bis-biotinylated DNA molecules with the tetravalent biotin-binding protein streptavidin (STV). By fitting the STV binding probabilities for the four possible valencies, the modelling correctly reproduces the dependencies of various network parameters experimentally observed in an earlier study. The combined results from the experimental and theoretical studies suggest that the binding probability for divalent STV formation is about 50 times larger than for the formation of trivalent and about 200 times larger than for tetravalent STV. In accordance with the experimental results, the modelling also indicates that the mixture of an equimolar ratio of DNA and STV leads to a maximum in size of the oligomeric DNA-STV clusters formed. Furthermore, we found a percolation transition in which the DNA cluster size increases rapidly with increasing DNA concentration resulting in the formation of a single supercluster at elevated concentrations. This behaviour coincides with the occurrence of an immobile band previously observed in electrophoretic experiments, indicating the formation of extremely large DNA-STV aggregate networks." @default.
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- W2012128325 date "2002-12-30" @default.
- W2012128325 modified "2023-10-12" @default.
- W2012128325 title "Monte Carlo Simulation of the Assembly of bis-Biotinylated DNA and Streptavidin" @default.
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- W2012128325 doi "https://doi.org/10.1002/cphc.200390012" @default.
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