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- W2012128686 abstract "Abstract.— A practical procedure for cryopreserving milt of paddlefish Polyodon sparhula was developed to obtain thawed spermatozoa that would fertilize eggs and permit hatching of normal larvae. Milt was mixed with a cryoprotectant medium containing DMSO (2.4 M) in a ratio of 3:1(milt: medium; final concentration of DMSO 0.6 M), stored in 5.0-mL freezing straws, and frozen in dry ice (15 min) and then in liquid nitrogen. A total of three replicates were made; the milt of a different male was used in each replicate. Motility of the thawed spermatozoa decreased to 50%-25% as compared to 100% motility of the fresh (control) spermatozoa. Hatching of paddlefish (16.3 ± 2.2%) from eggs fertilized with thawed spermatozoa was significantly lower (P≤ 0.01) than the hatch rate (90.8 ± 2.5%) for the control. It was suggested that an increase in viable motile spermatozoa to egg would result in better fertilization and hatching of paddlefish." @default.
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- W2012128686 date "1999-06-01" @default.
- W2012128686 modified "2023-10-03" @default.
- W2012128686 title "Cryopreservation of Paddlefish Polyodon spathula Milt" @default.
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- W2012128686 doi "https://doi.org/10.1111/j.1749-7345.1999.tb00871.x" @default.
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