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- W2012138768 abstract "A high-performance liquid chromatographic determination of two fenamates in human plasma is described. Plasma samples, 1.0 ml, to which 4 μg of internal standard had been added, were extracted with carbon tetrachloride under acidic conditions. Portions of the organic layer were transferred and evaporated to dryness under nitrogen. Residues were dissolved in methanol, and an aliquot was injected into the liquid chromatograph. An intermediate polarity, bonded cyanopropylsilane column was used with a mobile phase of water-acetonitrile-acetic acid (60:30:10 v/v/v). The flow rate was 1 ml/min, and the effluent was monitored at 254 nm. Flufenamic acid and mefenamic acid had retention times of 10.4 and 9.2 min, respectively. In the 1-10-μg range, the mean flufenamic acid recovery from control plasma was 100.7 ± 3.4% (n = 18). A typical calibration curve had a regression equation of y = 0.132x − 0.04 with γ2 = 0.99. Preliminary stability tests showed that flufenamic acid is stable for at least 2 weeks in plasma after freezing." @default.
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- W2012138768 date "1980-01-01" @default.
- W2012138768 modified "2023-10-14" @default.
- W2012138768 title "Determination of two Fenamates in Plasma by High-performance Liquid Chromatography" @default.
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- W2012138768 doi "https://doi.org/10.1002/jps.2600690128" @default.
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