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• W2012141738 abstract "Bulk and individual milk samples from 117 herds located in Brittany (west France) were used to assess: (i) the performance characteristics of an indirect enzyme-linked immunosorbent assay (ELISA) applied to individual milk for the detection of antibodies to bovine viral diarrhoea virus (BVDV); and (ii) the relationship between the bulk milk result obtained from this test and the within-herd prevalence of antibody-positive lactating cows. This ELISA test was based on a monoclonal antibody directed against non-structural protein NS2-3 of pestiviruses. At the individual level, based on 1113 matched milk/serum samples, the sensitivity and specificity of this test applied to milk, compared with the virus neutralization test on serum, were 95.0 and 97.7%, respectively. At the herd level, the relationship between the optical density percentage (OD%) of bulk milk and the within-herd prevalence of antibody-positive lactating cows was assessed using the receiver operating characteristics (ROC) analysis. Classes of OD% of bulk milk were determined so that they were associated with minimum intraclass and maximum between-class variances of within-herd prevalence of antibody-positive cows. The ROC analysis resulted in two classes of bulk milk results corresponding to different expected levels of within-herd prevalence. Herds with an OD% of bulk milk < 75% and > or = 75% had a mean observed prevalence of antibody-positive cows of 8.9 and 60.6%, respectively. Herds with a bulk milk result < 75% were expected to be BVDV free, whereas large variations in prevalence of antibody-positive cows existed in the herds with OD% > or = 75%. The test described in this study is suitable to identify herds likely to have a low prevalence of BVDV antibody-positive cows." @default.
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• W2012141738 date "2001-11-05" @default.
• W2012141738 modified "2023-10-17" @default.
• W2012141738 title "Informative Value of an Indirect Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Bovine Viral Diarrhoea Virus (BVDV) Antibodies in Milk" @default.
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• W2012141738 doi "https://doi.org/10.1046/j.1439-0450.2001.00497.x" @default.
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