Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012146451> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2012146451 abstract "The effects of insulin on glucose transport and metabolism were examined in cultured HT29 human colonic adenocarcinoma cells. The presence of glucose transporters was verified by D-glucose displaceable [3H]cytochalasin B binding. The Kd and Bmax values from cytochalasin B binding studies were 190 +/- 30 nM and 8.4 +/- 1.4 pmol/mg protein, respectively. Glucose transport determined with 3-O-methylglucose showed saturable kinetics with a Km of 5.8 +/- 0.4 mM and a Vmax of 0.047 +/- 0.003 mumol/mg protein per min at 25 degrees C. Moreover, in HT29 cells, two classes of insulin binding sites were detected in radioligand binding experiments. Although insulin failed to stimulate glucose transport, it was found to activate glycolysis in HT29 cells. Glucose consumption increased from 0.33 +/- 0.03 mumol/mg protein per h to 0.49 +/- 0.05 mumol/mg protein per h and lactate production was augmented from 0.67 +/- 0.04 mumol/mg protein per h to 0.87 +/- 0.06 mumol/mg protein per h in response to 10(-7) to 10(-5) M insulin. Insulin also enhanced mannose metabolism. Apart from these two hexoses, HT29 cells exhibited a surprisingly narrow substrate specificity. With the possible exception of glyceraldehyde, little lactate was produced from alternative substrates, including adenosine, inosine, ribose, deoxyribose, dihydroxyacetone, galactose and fructose either with or without insulin. Despite its limited utilization by the glycolytic pathway, adenosine was readily salvaged for de novo synthesis of adenine nucleotides. These findings suggest that insulin directly influences substrate utilization through the glycolytic pathway in HT29 cells without activating the glucose transport pathway." @default.
- W2012146451 created "2016-06-24" @default.
- W2012146451 creator A5016452935 @default.
- W2012146451 creator A5026343302 @default.
- W2012146451 creator A5027024129 @default.
- W2012146451 creator A5031435397 @default.
- W2012146451 date "1988-10-01" @default.
- W2012146451 modified "2023-09-24" @default.
- W2012146451 title "Insulin regulation of glucose metabolism in HT29 colonic adenocarcinoma cells: activation of glycolysis without augmentation of glucose transport" @default.
- W2012146451 cites W1434622850 @default.
- W2012146451 cites W1494018898 @default.
- W2012146451 cites W1513760428 @default.
- W2012146451 cites W1584900555 @default.
- W2012146451 cites W1604267989 @default.
- W2012146451 cites W2011031887 @default.
- W2012146451 cites W2012357829 @default.
- W2012146451 cites W2019087981 @default.
- W2012146451 cites W2025716683 @default.
- W2012146451 cites W2029772086 @default.
- W2012146451 cites W2029890054 @default.
- W2012146451 cites W2036641620 @default.
- W2012146451 cites W2039378659 @default.
- W2012146451 cites W2043060620 @default.
- W2012146451 cites W2047937128 @default.
- W2012146451 cites W2051380126 @default.
- W2012146451 cites W2065397874 @default.
- W2012146451 cites W2092740788 @default.
- W2012146451 cites W2134883783 @default.
- W2012146451 cites W2255703111 @default.
- W2012146451 cites W4251494161 @default.
- W2012146451 cites W75078479 @default.
- W2012146451 doi "https://doi.org/10.1016/0167-4889(88)90103-6" @default.
- W2012146451 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3052595" @default.
- W2012146451 hasPublicationYear "1988" @default.
- W2012146451 type Work @default.
- W2012146451 sameAs 2012146451 @default.
- W2012146451 citedByCount "9" @default.
- W2012146451 crossrefType "journal-article" @default.
- W2012146451 hasAuthorship W2012146451A5016452935 @default.
- W2012146451 hasAuthorship W2012146451A5026343302 @default.
- W2012146451 hasAuthorship W2012146451A5027024129 @default.
- W2012146451 hasAuthorship W2012146451A5031435397 @default.
- W2012146451 hasConcept C126322002 @default.
- W2012146451 hasConcept C134018914 @default.
- W2012146451 hasConcept C161573976 @default.
- W2012146451 hasConcept C17093226 @default.
- W2012146451 hasConcept C181199279 @default.
- W2012146451 hasConcept C185592680 @default.
- W2012146451 hasConcept C20251656 @default.
- W2012146451 hasConcept C202751555 @default.
- W2012146451 hasConcept C2776317432 @default.
- W2012146451 hasConcept C2776970464 @default.
- W2012146451 hasConcept C2777866211 @default.
- W2012146451 hasConcept C2779012151 @default.
- W2012146451 hasConcept C2779306644 @default.
- W2012146451 hasConcept C2779735320 @default.
- W2012146451 hasConcept C2780881558 @default.
- W2012146451 hasConcept C2781272698 @default.
- W2012146451 hasConcept C55493867 @default.
- W2012146451 hasConcept C62231903 @default.
- W2012146451 hasConcept C71924100 @default.
- W2012146451 hasConcept C86803240 @default.
- W2012146451 hasConceptScore W2012146451C126322002 @default.
- W2012146451 hasConceptScore W2012146451C134018914 @default.
- W2012146451 hasConceptScore W2012146451C161573976 @default.
- W2012146451 hasConceptScore W2012146451C17093226 @default.
- W2012146451 hasConceptScore W2012146451C181199279 @default.
- W2012146451 hasConceptScore W2012146451C185592680 @default.
- W2012146451 hasConceptScore W2012146451C20251656 @default.
- W2012146451 hasConceptScore W2012146451C202751555 @default.
- W2012146451 hasConceptScore W2012146451C2776317432 @default.
- W2012146451 hasConceptScore W2012146451C2776970464 @default.
- W2012146451 hasConceptScore W2012146451C2777866211 @default.
- W2012146451 hasConceptScore W2012146451C2779012151 @default.
- W2012146451 hasConceptScore W2012146451C2779306644 @default.
- W2012146451 hasConceptScore W2012146451C2779735320 @default.
- W2012146451 hasConceptScore W2012146451C2780881558 @default.
- W2012146451 hasConceptScore W2012146451C2781272698 @default.
- W2012146451 hasConceptScore W2012146451C55493867 @default.
- W2012146451 hasConceptScore W2012146451C62231903 @default.
- W2012146451 hasConceptScore W2012146451C71924100 @default.
- W2012146451 hasConceptScore W2012146451C86803240 @default.
- W2012146451 hasLocation W20121464511 @default.
- W2012146451 hasLocation W20121464512 @default.
- W2012146451 hasOpenAccess W2012146451 @default.
- W2012146451 hasPrimaryLocation W20121464511 @default.
- W2012146451 hasRelatedWork W1979204365 @default.
- W2012146451 hasRelatedWork W2006513370 @default.
- W2012146451 hasRelatedWork W2080438079 @default.
- W2012146451 hasRelatedWork W2081817671 @default.
- W2012146451 hasRelatedWork W2086917972 @default.
- W2012146451 hasRelatedWork W2096423991 @default.
- W2012146451 hasRelatedWork W2281673292 @default.
- W2012146451 hasRelatedWork W2324496841 @default.
- W2012146451 hasRelatedWork W2395688104 @default.
- W2012146451 hasRelatedWork W2410784692 @default.
- W2012146451 isParatext "false" @default.
- W2012146451 isRetracted "false" @default.
- W2012146451 magId "2012146451" @default.
- W2012146451 workType "article" @default.