Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012147305> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2012147305 endingPage "27" @default.
- W2012147305 startingPage "17" @default.
- W2012147305 abstract "To investigate the intracellular localisation and biological activity of procorticotrophin-releasing hormone (proCRH), we have established stably transfected CHO-K1 cells expressing the rat pre-proCRH cDNA. Using immunoblot analysis of cell lysates of transfected CHO-K1 cells, we detected a major CRH immunoreactive band with an apparent molecular weight of approximately 19 kDa. This 19 kDa band could account for full length proCRH molecule which has not undergone post-translational modifications. Metabolic labelling followed by immunoprecipitation, SDS-PAGE and autoradiography indicated that no endoproteolytic processing of proCRH takes place within the transfected CHO-K1 cells. Immunofluorescence staining localises the CRH precursor to both the cytoplasm and to the nucleus in transfected CHO-K1 cells. This result was confirmed using subcellular fractionation techniques on radiolabelled CHO-K1 cells expressing immunoreactive CRH. A major CRH-immunoreactive band of 19 kDa was detected both in the microsomal and secreted fractions, indicating the presence of proCRH within the secretory pathway of these cells. This was also evident in the nuclear fraction, therefore confirming the nuclear localisation of proCRH. Analysis of DNA concentration, cell number and DNA synthesis showed that stably transfected CHO-K1 cells expressing proCRH have a higher proliferation and DNA synthesis rate than wildtype CHO-K1 cells or CHO-K1 cells transfected with pEE14 alone. Our results therefore suggest a mitogenic role for the intact proCRH molecule within CHO-K1 cells. Furthermore, treatment of mouse corticotrophic tumour cells (AtT20/D16-16) with conditioned medium from transfected CHO-K1 cells expressing proCRH, stimulated both DNA synthesis and cell proliferation above basal levels. Our results constitute the first reported direct evidence of a mitogenic role for proCRH acting on a corticotrophic cell population." @default.
- W2012147305 created "2016-06-24" @default.
- W2012147305 creator A5001815156 @default.
- W2012147305 creator A5018240758 @default.
- W2012147305 creator A5020057911 @default.
- W2012147305 creator A5020454445 @default.
- W2012147305 creator A5051700610 @default.
- W2012147305 creator A5054277909 @default.
- W2012147305 creator A5076574165 @default.
- W2012147305 creator A5078577158 @default.
- W2012147305 creator A5080277287 @default.
- W2012147305 date "1995-01-01" @default.
- W2012147305 modified "2023-09-25" @default.
- W2012147305 title "Mitogenic effects and nuclear localisation of procorticotrophin-releasing hormone expressed within stably transfected fibroblast cells (CHO-K1)" @default.
- W2012147305 cites W1480056716 @default.
- W2012147305 cites W1966603679 @default.
- W2012147305 cites W1975849939 @default.
- W2012147305 cites W2004037805 @default.
- W2012147305 cites W2018791991 @default.
- W2012147305 cites W2023023053 @default.
- W2012147305 cites W2032003686 @default.
- W2012147305 cites W2042996310 @default.
- W2012147305 cites W2052438486 @default.
- W2012147305 cites W2056685323 @default.
- W2012147305 cites W2065232041 @default.
- W2012147305 cites W2065876190 @default.
- W2012147305 cites W2075065147 @default.
- W2012147305 cites W2079583639 @default.
- W2012147305 cites W2080096872 @default.
- W2012147305 cites W2081082423 @default.
- W2012147305 cites W2089844518 @default.
- W2012147305 cites W2090048653 @default.
- W2012147305 cites W2150211362 @default.
- W2012147305 cites W2157397275 @default.
- W2012147305 cites W2159090199 @default.
- W2012147305 cites W2175970545 @default.
- W2012147305 doi "https://doi.org/10.1016/0303-7207(94)03416-q" @default.
- W2012147305 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7796932" @default.
- W2012147305 hasPublicationYear "1995" @default.
- W2012147305 type Work @default.
- W2012147305 sameAs 2012147305 @default.
- W2012147305 citedByCount "17" @default.
- W2012147305 crossrefType "journal-article" @default.
- W2012147305 hasAuthorship W2012147305A5001815156 @default.
- W2012147305 hasAuthorship W2012147305A5018240758 @default.
- W2012147305 hasAuthorship W2012147305A5020057911 @default.
- W2012147305 hasAuthorship W2012147305A5020454445 @default.
- W2012147305 hasAuthorship W2012147305A5051700610 @default.
- W2012147305 hasAuthorship W2012147305A5054277909 @default.
- W2012147305 hasAuthorship W2012147305A5076574165 @default.
- W2012147305 hasAuthorship W2012147305A5078577158 @default.
- W2012147305 hasAuthorship W2012147305A5080277287 @default.
- W2012147305 hasConcept C153911025 @default.
- W2012147305 hasConcept C175656101 @default.
- W2012147305 hasConcept C181199279 @default.
- W2012147305 hasConcept C190062978 @default.
- W2012147305 hasConcept C27239569 @default.
- W2012147305 hasConcept C54009773 @default.
- W2012147305 hasConcept C54355233 @default.
- W2012147305 hasConcept C55493867 @default.
- W2012147305 hasConcept C71829478 @default.
- W2012147305 hasConcept C81885089 @default.
- W2012147305 hasConcept C86803240 @default.
- W2012147305 hasConcept C95444343 @default.
- W2012147305 hasConceptScore W2012147305C153911025 @default.
- W2012147305 hasConceptScore W2012147305C175656101 @default.
- W2012147305 hasConceptScore W2012147305C181199279 @default.
- W2012147305 hasConceptScore W2012147305C190062978 @default.
- W2012147305 hasConceptScore W2012147305C27239569 @default.
- W2012147305 hasConceptScore W2012147305C54009773 @default.
- W2012147305 hasConceptScore W2012147305C54355233 @default.
- W2012147305 hasConceptScore W2012147305C55493867 @default.
- W2012147305 hasConceptScore W2012147305C71829478 @default.
- W2012147305 hasConceptScore W2012147305C81885089 @default.
- W2012147305 hasConceptScore W2012147305C86803240 @default.
- W2012147305 hasConceptScore W2012147305C95444343 @default.
- W2012147305 hasIssue "1" @default.
- W2012147305 hasLocation W20121473051 @default.
- W2012147305 hasLocation W20121473052 @default.
- W2012147305 hasOpenAccess W2012147305 @default.
- W2012147305 hasPrimaryLocation W20121473051 @default.
- W2012147305 hasRelatedWork W2012147305 @default.
- W2012147305 hasRelatedWork W2053393868 @default.
- W2012147305 hasRelatedWork W2111556389 @default.
- W2012147305 hasRelatedWork W2337696957 @default.
- W2012147305 hasRelatedWork W2358953894 @default.
- W2012147305 hasRelatedWork W2363104495 @default.
- W2012147305 hasRelatedWork W2378943006 @default.
- W2012147305 hasRelatedWork W2391130556 @default.
- W2012147305 hasRelatedWork W2442342451 @default.
- W2012147305 hasRelatedWork W2117818578 @default.
- W2012147305 hasVolume "107" @default.
- W2012147305 isParatext "false" @default.
- W2012147305 isRetracted "false" @default.
- W2012147305 magId "2012147305" @default.
- W2012147305 workType "article" @default.