Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012150809> ?p ?o ?g. }
- W2012150809 endingPage "998" @default.
- W2012150809 startingPage "985" @default.
- W2012150809 abstract "Numerous membrane proteins are cleaved by tumor necrosis factor-alpha converting enzyme (TACE), which causes the release of their ectodomains. An ADAM (a disintegrin and metalloprotease domain) family member, TACE contains several noncatalytic domains whose roles in ectodomain shedding have yet to be fully resolved. Here, we have explored the function of the transmembrane domain (TM) of TACE by coupling molecular engineering and functional analysis. A TM-free TACE construct that is anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI)-binding polypeptide failed to restore shedding of transforming growth factor-alpha (TGF-alpha), tumor necrosis factor-alpha (TNF-alpha) and L-selectin in cells lacking endogenous TACE activity. Substitution of the TACE TM with that of the prolactin receptor or platelet-derived growth factor receptor (PDGFR) also resulted in severe loss of TGF-alpha shedding, but had no effects on the cleavage of TNF-alpha and L-selectin. Replacement of the TM in TGF-alpha with that of L-selectin enabled TGF-alpha shedding by the TACE mutants carrying the TM of prolactin receptor and PDGFR. Taken together, our observations suggest that anchorage of TACE to the lipid bilayer through a TM is required for efficient cleavage of a broad spectrum of substrates, and that the amino-acid sequence of TACE TM may play a role in regulatory specificity among TACE substrates." @default.
- W2012150809 created "2016-06-24" @default.
- W2012150809 creator A5016630625 @default.
- W2012150809 creator A5025549159 @default.
- W2012150809 creator A5070233280 @default.
- W2012150809 creator A5089610748 @default.
- W2012150809 date "2007-11-27" @default.
- W2012150809 modified "2023-10-11" @default.
- W2012150809 title "The transmembrane domain of TACE regulates protein ectodomain shedding" @default.
- W2012150809 cites W1582119606 @default.
- W2012150809 cites W1607068798 @default.
- W2012150809 cites W1959148860 @default.
- W2012150809 cites W1960631461 @default.
- W2012150809 cites W1966481456 @default.
- W2012150809 cites W1967485093 @default.
- W2012150809 cites W1968456917 @default.
- W2012150809 cites W1975614676 @default.
- W2012150809 cites W1984632140 @default.
- W2012150809 cites W1995215171 @default.
- W2012150809 cites W1999264521 @default.
- W2012150809 cites W2002765492 @default.
- W2012150809 cites W2010883488 @default.
- W2012150809 cites W2036631165 @default.
- W2012150809 cites W2036799673 @default.
- W2012150809 cites W2036842448 @default.
- W2012150809 cites W2036851890 @default.
- W2012150809 cites W2039278431 @default.
- W2012150809 cites W2042087846 @default.
- W2012150809 cites W2044157798 @default.
- W2012150809 cites W2044312475 @default.
- W2012150809 cites W2047694858 @default.
- W2012150809 cites W2048264270 @default.
- W2012150809 cites W2056943748 @default.
- W2012150809 cites W2067450274 @default.
- W2012150809 cites W2067928188 @default.
- W2012150809 cites W2071607446 @default.
- W2012150809 cites W2074721095 @default.
- W2012150809 cites W2074881372 @default.
- W2012150809 cites W2075840864 @default.
- W2012150809 cites W2076233716 @default.
- W2012150809 cites W2078023329 @default.
- W2012150809 cites W2078269233 @default.
- W2012150809 cites W2079096224 @default.
- W2012150809 cites W2079474526 @default.
- W2012150809 cites W2093159369 @default.
- W2012150809 cites W2102006131 @default.
- W2012150809 cites W2113359881 @default.
- W2012150809 cites W2119445810 @default.
- W2012150809 cites W2122882997 @default.
- W2012150809 cites W2123343744 @default.
- W2012150809 cites W2125656357 @default.
- W2012150809 cites W2127669953 @default.
- W2012150809 cites W2133034461 @default.
- W2012150809 cites W2134564419 @default.
- W2012150809 cites W2137033699 @default.
- W2012150809 cites W2138892164 @default.
- W2012150809 cites W2166612164 @default.
- W2012150809 cites W2318843727 @default.
- W2012150809 doi "https://doi.org/10.1038/cr.2007.98" @default.
- W2012150809 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18040288" @default.
- W2012150809 hasPublicationYear "2007" @default.
- W2012150809 type Work @default.
- W2012150809 sameAs 2012150809 @default.
- W2012150809 citedByCount "25" @default.
- W2012150809 countsByYear W20121508092012 @default.
- W2012150809 countsByYear W20121508092013 @default.
- W2012150809 countsByYear W20121508092015 @default.
- W2012150809 countsByYear W20121508092017 @default.
- W2012150809 countsByYear W20121508092018 @default.
- W2012150809 countsByYear W20121508092021 @default.
- W2012150809 countsByYear W20121508092022 @default.
- W2012150809 countsByYear W20121508092023 @default.
- W2012150809 crossrefType "journal-article" @default.
- W2012150809 hasAuthorship W2012150809A5016630625 @default.
- W2012150809 hasAuthorship W2012150809A5025549159 @default.
- W2012150809 hasAuthorship W2012150809A5070233280 @default.
- W2012150809 hasAuthorship W2012150809A5089610748 @default.
- W2012150809 hasBestOaLocation W20121508091 @default.
- W2012150809 hasConcept C1009742 @default.
- W2012150809 hasConcept C118892022 @default.
- W2012150809 hasConcept C125555471 @default.
- W2012150809 hasConcept C134018914 @default.
- W2012150809 hasConcept C153911025 @default.
- W2012150809 hasConcept C170493617 @default.
- W2012150809 hasConcept C17991360 @default.
- W2012150809 hasConcept C181199279 @default.
- W2012150809 hasConcept C24530287 @default.
- W2012150809 hasConcept C2776362946 @default.
- W2012150809 hasConcept C2778259572 @default.
- W2012150809 hasConcept C2778715156 @default.
- W2012150809 hasConcept C55493867 @default.
- W2012150809 hasConcept C55728118 @default.
- W2012150809 hasConcept C86803240 @default.
- W2012150809 hasConcept C95444343 @default.
- W2012150809 hasConceptScore W2012150809C1009742 @default.
- W2012150809 hasConceptScore W2012150809C118892022 @default.
- W2012150809 hasConceptScore W2012150809C125555471 @default.
- W2012150809 hasConceptScore W2012150809C134018914 @default.