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• W2012160237 abstract "Adult stem cells are nowadays used for treating several pathologies. A putative stem cell population was found in the adipose tissue of mammals and canine adipose tissue-derived-mesenchymal stem cells (cA-MSC) have been shown to possess the capacity to differentiate into several lineages. The main goal of our research was to fully characterize cA-MSC and examine the effects of cryopreservation on their stemness features. Each sample of cA-MSC was analyzed immediately and then again after being frozen in liquid nitrogen for one year. After the cryopreservation period cells conserved their fibroblast-like morphology, alkaline phosphatase positivity and CD expression but showed a lower proliferation ratio and a lower telomerase activity in comparison with fresh cells. Finally, the cryopreservation protocol did not change the cA-MSC adipogenic, osteogenic and myogenic differentiative potential. Our data demonstrate that stored cA-MSC might represent a promising type of progenitor cell for autologous cellular-based therapies in veterinary medicine." @default.
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• W2012160237 date "2011-08-01" @default.
• W2012160237 modified "2023-09-27" @default.
• W2012160237 title "Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation" @default.
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• W2012160237 doi "https://doi.org/10.1016/j.rvsc.2010.07.024" @default.
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